日韩午夜在线观看,色偷偷伊人,免费一级毛片不卡不收费,日韩午夜在线视频不卡片

齊一生物科技(上海)有限公司

小鼠胰島素(INS)酶聯(lián)免疫試劑盒-齊一生物

時(shí)間:2018-3-15閱讀:400
分享:

For Research Use Only

僅供研究用

貨號(hào):QY-M30038

小鼠胰島素(INS)酶聯(lián)免疫分析(ELISA)

試劑盒使用說明書

 

本試劑僅供研究使用 目的:本試劑盒用于測定小鼠血清,細(xì)胞上清及相關(guān)液體樣本中胰島素(INS)的含量。

實(shí)驗(yàn)原理:

本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中小鼠胰島素(INS)水平。用純化的小鼠抗-胰島素(INS)抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入胰島素(INS),再與 HRP 標(biāo)記的胰島素(INS)抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過*洗滌后加底物 TMB 顯色。TMB  HRP 酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的胰島素(INS)呈正相關(guān)。用酶標(biāo)儀在 450nm 波長下測定吸光度(OD 值),通過標(biāo)準(zhǔn)曲線計(jì)算樣品中小鼠胰島素(INS)濃度。

Specimen requirements

N

Assay procedure

 

1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separay. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 12 mU/L,8mU/L ,4 mU/L,2 mU/L, 1 mU/L)

2.add sample  Set blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.

3.add enzymeAdd HRP-Conjugate reagent 50μl to each well, except blank well.

4.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37. 5.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.

6.washingUncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

7.colorAdd Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 10 min at 37

8.Stop the reaction  Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

9.assaytake blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.

Important notes

  • The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
  • washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
  • add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
  • if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.×n×5.
  • Closure plate membrane only limits the disposable use, to avoid cross-contamination.
  • The substrate evade the light preservation.
  • Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
  • All samples, washing buffer and each kind of reject should according to infective material process.
  • Do not mix reagents with those from other lots.

Assay range

0.3 mU/L -15 mU/L

 

Storage and validity

1Storage 2-8.

2validity six months.

齊一生物科技(上海)有限公司主要經(jīng)營進(jìn)口原裝Elisa試劑盒,自產(chǎn)品牌QIYBO試劑盒,并提供免疫分析相關(guān)試劑和檢測試劑盒。種類齊全,價(jià)格實(shí)惠,凡購買我公司產(chǎn)品的新老客戶,即可享免費(fèi)代測實(shí)驗(yàn)服務(wù),可檢測多種屬樣本。全程提供。如需其他Elisa試劑盒說明書及報(bào)價(jià),可我公司! 

會(huì)員登錄

×

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),環(huán)保在線對(duì)此不承擔(dān)任何保證責(zé)任。

溫馨提示:為規(guī)避購買風(fēng)險(xiǎn),建議您在購買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

在線留言
主站蜘蛛池模板: 璧山县| 台安县| 甘泉县| 隆化县| 汾阳市| 临西县| 车险| 鲁甸县| 清水县| 福海县| 柘荣县| 繁昌县| 新兴县| 兴国县| 祁东县| 资溪县| 晋城| 盐津县| 万州区| 福泉市| 太保市| 寿宁县| 繁昌县| 临西县| 泗阳县| 岗巴县| 晋中市| 肇州县| 如东县| 太原市| 蓝田县| 文化| 凯里市| 蓬安县| 东平县| 高阳县| 遵义县| 高唐县| 德令哈市| 安龙县| 连山|