G418二硫酸鹽
分類 其它生化試劑
級別 優級純GR
產品規格 SIGMA A1720-1G
CAS 108321-42-2
品牌 SIGMA
用途范圍 生物科研
保存溫度 2-8℃
分子式 C20H40N4O10 · 2H2SO4
分子量 692.71
供應商 上海研伴實業有限公司
G418二硫酸鹽 SIGMA A1720-1G
powder, BioReagent, suitable for cell culture
· 產品編號：A1720
· 中文名稱：G418 二硫酸鹽
· 英文名稱：G418 disulfate salt
· CAS號：108321-42-2
· 分子式：C20H40N4O10 · 2H2SO4
· 分子量：692.71
· 保存溫度：2-8 °C
· MDL號：MFCD00058314
訂貨信息：
產品規格價格(RMB)
· A1720-1G 電儀/元
· A1720-5G 電儀/元
· A1720-25G 電儀/元
注：此價格僅供參考，請以SIGMA價格為準，優惠價請電詢。
性能描述
Biochem/physiol Actions
G 418 blocks polypeptide synthesis by interfering with ribosomal function and inhibiting protein elongation.
Mode of Action: Blocks polypeptide synthesis by inhibiting protein elongation. For use in the selection and maintenance of eukaryotic cells stably transfected with neomycin resistance genes.
Mode of Action: Blocks polypeptide synthesis by inhibiting protein synthesis at the level of the 70S and 80S ribosomes. For use in the selection and maintenance of eukaryotic cells stably transfected with neomycin resistance genes.
Other Notes
Aminoglycoside antibiotic similar in structure to gentamicin, neomycin and kanamycin.
Application
G 418 is a Micromonospora-produced aminoglycoside antibiotic with activity against protozoa and helminths1. G 418 disulfate salt is used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene2.
G 418 is an aminoglycoside antibiotic similar in structure to gentamycin. It is normally toxic towards eukaryotic and prokaryotic cells, but is useful for selection of cells stably transfected with neomycin resistance gene (Neo). The optimal concentration for selection and maintenance must be determined for each cell line. For bacteria and algae, concentrations of 5 μg/ml or less are recommended. Animal cells may require up to 300-500 μg/ml. Typically, resistance is conferred by one of two dominant genes of bacterial origin, which can be expressed in eukaryotic cells. Cells that are multiplying will be affected sooner than those are quiescent. Cells in log phase may require three to seven days for selection. In general, concentrations of approximay 400 μg/ml for selection and 200 μg/ml for maintenance are required for mammalian cells.
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