上海研盟生物有限公司*品質(zhì)供應(yīng)Anti-Keratin82抗體,我公司銷(xiāo)售國(guó)內(nèi)外科研產(chǎn)品,如生物試劑、生化試劑,一抗二抗,各種標(biāo)記抗體,Elisa酶聯(lián)免疫試劑盒,放免試劑盒,培養(yǎng)基,各種動(dòng)物血清等產(chǎn)品,歡迎廣大新老客戶(hù)前來(lái)咨詢(xún)訂購(gòu),訂購(gòu):或。
中文名稱(chēng):角蛋白82抗體
英文名稱(chēng):Anti-Keratin82抗體
避免反復(fù)凍融,保質(zhì)期一年。
角蛋白82抗體,Anti-Keratin82抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
各種HRP酶標(biāo)記成抗體的效價(jià)測(cè)定程序如下(如:羊抗鼠IgG-HRP的效價(jià)測(cè)定):
1、抗原包板:15ug/ml 小鼠IgG-pH9.6·CB液 0.1ml/孔;
2、37°C包被60min或4°C過(guò)夜;
3、PBST洗板3次,5min/次;
4、加入倍比稀釋的HRP標(biāo)記抗體 0.1ml/孔;
5、37°C 30min;
6、PBST洗板5min/次×3;
7、底物液0.1ml/孔 37°C 5-10min;
8、終止液:2N H2SO4 0.05ml/孔;
9、450nm 測(cè)定OD值;
10、陽(yáng)性O(shè)D值比對(duì)照值大3倍,為判斷效價(jià)稀釋度。
角蛋白82抗體,Anti-Keratin82抗體簡(jiǎn)單介紹
別名:Hard keratin, type II, 2; Hb 2; HB2; K82; Kb22; Keratin 82; Keratin hair basic 2; Keratin, type II cuticular Hb2; Krt2 20; Krt82; KRTHB2; Type II hair keratin Hb2; Type II keratin Kb22; KRT82_HUMAN.
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來(lái)源:本公司抗體有兔來(lái)源和鼠來(lái)源等
克隆類(lèi)型:兔來(lái)源為多抗,鼠來(lái)源為單抗
產(chǎn)品類(lèi)型:一抗
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
角蛋白82抗體背景介紹:KRT82 is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this keratin appears to be a hair cuticle-specific keratin.
免疫熒光染色與免疫組化前期處理相同,因?yàn)閮?nèi)源性過(guò)氧化物酶對(duì)免疫熒光實(shí)驗(yàn)沒(méi)有影響,同時(shí)雙氧水反而會(huì)干擾到熒光素發(fā)光,所以這步操作在免疫熒光中不需要做。
常用的熒光顯微鏡一般有三個(gè)通路,激發(fā)波長(zhǎng)為350nm、488nm、555nm附近,分別對(duì)應(yīng)的熒光發(fā)射光顏色為藍(lán)光、綠光、紅光。
350nm常用熒光素:DAPI(復(fù)染用),Alexa Fluor 350
488nm常用熒光素:FITC,Alexa Fluor 488
555nm常用熒光素:PE、Cy3、Rhodamine B、Alexa Fluor555
另有其他常見(jiàn)熒光素
594nm常用熒光素:Texas Red、Alexa Fluor594
647nm常用熒光素:Cy5、APC、Alexa Fluor647
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