上海研盟生物有限公司*品質供應Anti-APBA2抗體,我公司銷售國內外科研產品,如生物試劑、生化試劑,一抗二抗,各種標記抗體,Elisa酶聯免疫試劑盒,放免試劑盒,培養基,各種動物血清等產品,歡迎廣大新老客戶前來咨詢訂購,訂購:或。
中文名稱:β淀粉樣前體蛋白結合蛋白2抗體(X11β)
英文名稱:Anti-APBA2抗體
避免反復凍融,保質期一年。
β淀粉樣前體蛋白結合蛋白2抗體,Anti-APBA2抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
各種HRP酶標記成抗體的效價測定程序如下(如:羊抗鼠IgG-HRP的效價測定):
1、抗原包板:15ug/ml 小鼠IgG-pH9.6·CB液 0.1ml/孔;
2、37°C包被60min或4°C過夜;
3、PBST洗板3次,5min/次;
4、加入倍比稀釋的HRP標記抗體 0.1ml/孔;
5、37°C 30min;
6、PBST洗板5min/次×3;
7、底物液0.1ml/孔 37°C 5-10min;
8、終止液:2N H2SO4 0.05ml/孔;
9、450nm 測定OD值;
10、陽性OD值比對照值大3倍,為判斷效價稀釋度。
β淀粉樣前體蛋白結合蛋白2抗體,Anti-APBA2抗體簡單介紹
別名:Adapter protein X11beta; Amyloid beta A4 precursor protein-binding family A member 2; APBA2; APBA2_HUMAN; D15S1518E; HsT16821; LIN 10; Mint 2; Mint-2; Neuron-specific X11L protein; Neuronal Munc18-1-interacting protein 2; Phosphotyrosine binding/interacting domain (PTB) bearing protein; X11 beta; X11l.
規格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來源:本公司抗體有兔來源和鼠來源等
克隆類型:兔來源為多抗,鼠來源為單抗
產品類型:一抗
此產品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
β淀粉樣前體蛋白結合蛋白2抗體背景介紹:The Beta-Amyloid precursor protein (Beta-APP) is a major constituent of the amyloid deposits in patients with Alzheimer’s disease. The Beta-Amyloid precursor is known to interact with several proteins, including X11 and the G heterotrimetric protein APP-BP1. The neuronal, transmembrane protein X11 is known to bind to the ∫-Amyloid precursor protein via a phosphotyrosine binding (PTB) domain, reducing the secretion of cellular Beta-APP and slowing Beta-APP processing pathways. X11 binds specifically to the YENPTY motif, which is involved in the internalization of Beta-APP. Multiple splice varitents of X11 have been identified, including X11 Alpha (also designated Mint 1), X11 Beta (Mint 2) and X11 Gamma(Mint 3).
免疫熒光染色與免疫組化前期處理相同,因為內源性過氧化物酶對免疫熒光實驗沒有影響,同時雙氧水反而會干擾到熒光素發光,所以這步操作在免疫熒光中不需要做。
常用的熒光顯微鏡一般有三個通路,激發波長為350nm、488nm、555nm附近,分別對應的熒光發射光顏色為藍光、綠光、紅光。
350nm常用熒光素:DAPI(復染用),Alexa Fluor 350
488nm常用熒光素:FITC,Alexa Fluor 488
555nm常用熒光素:PE、Cy3、Rhodamine B、Alexa Fluor555
另有其他常見熒光素
594nm常用熒光素:Texas Red、Alexa Fluor594
647nm常用熒光素:Cy5、APC、Alexa Fluor647
β淀粉樣前體蛋白結合蛋白2抗體,Anti-APBA2抗體相關優質產品:
Mfn1 (Mitofusin1) 線粒體融合蛋白1抗體
MGMT (O6-metYMlguanine-DNA metYMltransferase) O6甲基*DNA甲基轉移酶抗體
MT(metallothionein) 金屬硫蛋白抗體
MGr1-Ag/37LRP(P37-kDa laminin receptor precuBYKor)(NT) 層粘連蛋白受體1抗體(N端)
MTA1(Metastasis-associated 1) 腫瘤轉移相關蛋白1抗體
MGr1-Ag/37LRP(P37-kDa laminin receptor precuBYKor)(CT) 層粘連蛋白受體1抗體(C端)
Myf6(Myogenic factor 6) 生肌調節因子6抗體
MGr1-Ag monoclonal antibody(laminin receptor 1; 67kD, ribosomal protein SA) 小鼠抗層粘連蛋白受體1單克隆抗體
MICA(MHC class I polypeptide-related sequence A) MHC I類鏈相關蛋白A抗體
Microsporidia 蜜蜂微孢子蟲蛋白抗體
Midnolin isoform Protein 1 中腦核仁蛋白1抗體
Midnolin isoform Protein 2 中腦核仁蛋白2抗體
MK(Midkine) 中期因子/肝素結合生長因子抗體
MLCK(myosin light chain kinase) 肌球蛋白輕鏈激酶抗體
MIF (Macrophage Migration Inhibitory Factor) 巨噬細胞移動抑制因子抗體
MIP-1α (macrophage inflammatory protein 1α) 巨噬細胞炎癥因子1α抗體
MIP-1β (macrophage inflammatory protein 1β) 巨噬細胞炎癥因子1β 抗體
MIP4/CCL18(Macrophage inflammatory protein 4) 巨噬細胞炎癥蛋白18抗體