磺酸基-Cy7-馬來(lái)酰亞胺，Sulfo-Cy7-Mal，水溶性熒光染料
A reactive dye with a maleimide group for the labeling of thiol groups of proteins and other biomolecules, an analog of Cy7® maleimide.
Sulfo-Cyanine7 is a near infrared fluorophore which is hydrophilic and contains a rigidizing ring in its heptamethine chain that improves its photophysical properties. A great dye for various applications, including NIR imaging.
供應(yīng)商：西安凱新生物科技有限公司
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General properties
Appearance: | dark green powder |
Mass spec M+ increment: | 868.2 |
Molecular weight: | 869.10 |
Molecular formula: | C43H49KN4O9S2 |
IUPAC name: | 1-[2-(6-{2-[(E)-2-[(E)-3-{(E)-2-[1-Methyl-3,3-dimethyl-5-(oxysulfonyl)-2-indolinylidene]ethylidene}-1-cyclohexen-1-yl]ethenyl]-3,3-dimethyl-5-(oxysulfonyl)-3H-indol-1-yl}hexanoylamino)ethyl]-1H-pyrrole-2,5-dione, potassium salt |
Solubility: | Good in water, DMF, DMSO. Low in non-polar organic solvents. |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
Excitation maximum, nm: | 750 |
ε, L⋅mol−1⋅cm−1: | 240600 |
Emission maximum, nm: | 773 |
CF260: | 0.04 |
CF280: | 0.04 |
菁染料是性能優(yōu)良的熒光標(biāo)記染料，摩爾吸光系數(shù)在熒光染料中是zui高的，其琥珀酰亞胺酯是zui常用的脂肪氨基標(biāo)記試劑，廣泛用于蛋白、抗體、核酸及其他生物分子的標(biāo)記和檢測(cè)。通過(guò)改變次甲基鏈的長(zhǎng)度, 可改變其熒光發(fā)射波長(zhǎng)，每增加一個(gè)雙鍵，按照Huoffman 規(guī)則正好紅移約100nm。
菁染料Cy3 和Cy5 已成為基因芯片的熒光標(biāo)記物; 另外,Cy5, Cy5.5 和Cy7 的吸收在近紅外區(qū)背景非常低，是熒光強(qiáng)度zui高、zui穩(wěn)定的長(zhǎng)波長(zhǎng)染料。特別適合于活體小動(dòng)物體內(nèi)成像代替放射性元素。
但由于菁染料, 尤其是不對(duì)稱(chēng)菁染料的合成副反應(yīng)多, 副產(chǎn)物極性相近, 產(chǎn)物的分離提純相當(dāng)困難。菁染料，特別是水溶性菁染料分子極性大, 分離提純?cè)郊永щy
磺酸基-Cy7-馬來(lái)酰亞胺，Sulfo-Cy7-Mal，水溶性熒光染料
