水溶性近紅外熒光染料Sulfo-Cy7 maleimide
A reactive dye with a maleimide group for the labeling of thiol groups of proteins and other biomolecules, an analog of Cy7® maleimide.
Sulfo-Cyanine7 is a near infrared fluorophore which is hydrophilic and contains a rigidizing ring in its heptamethine chain that improves its photophysical properties. A great dye for various applications, including NIR imaging.
General properties
Appearance: | dark green powder |
Mass spec M+ increment: | 868.2 |
Molecular weight: | 869.10 |
Molecular formula: | C43H49KN4O9S2 |
IUPAC name: | 1-[2-(6-{2-[(E)-2-[(E)-3-{(E)-2-[1-Methyl-3,3-dimethyl-5-(oxysulfonyl)-2-indolinylidene]ethylidene}-1-cyclohexen-1-yl]ethenyl]-3,3-dimethyl-5-(oxysulfonyl)-3H-indol-1-yl}hexanoylamino)ethyl]-1H-pyrrole-2,5-dione, potassium salt |
Solubility: | Good in water, DMF, DMSO. Low in non-polar organic solvents. |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
Excitation maximum, nm: | 750 |
ε, L⋅mol−1⋅cm−1: | 240600 |
Emission maximum, nm: | 773 |
CF260: | 0.04 |
CF280: | 0.04 |
菁染料是性能優良的熒光標記染料，摩爾吸光系數在熒光染料中是zui高的，其琥珀酰亞胺酯是zui常用的脂肪氨基標記試劑，廣泛用于蛋白、抗體、核酸及其他生物分子的標記和檢測。通過改變次甲基鏈的長度, 可改變其熒光發射波長，每增加一個雙鍵，按照Huoffman 規則正好紅移約100nm。
菁染料Cy3 和Cy5 已成為基因芯片的熒光標記物; 另外,Cy5, Cy5.5 和Cy7 的吸收在近紅外區背景非常低，是熒光強度zui高、zui穩定的長波長染料。特別適合于活體小動物體內成像代替放射性元素。
但由于菁染料, 尤其是不對稱菁染料的合成副反應多, 副產物極性相近, 產物的分離提純相當困難。菁染料，特別是水溶性菁染料分子極性大, 分離提純越加困難
供應商：西安凱新生物科技有限公司
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其它馬來酰亞胺類染料 Dye maleimides：
BDP FL 馬來酰亞胺酯 BDP FL maleimide
BDP FL maleimide is a bright and photostable thiol-reactive dye for protein labeling, an ideal replacement for fluorescein for microscopy.
Cy3-馬來酰亞胺酯Cyanine3 maleimide
Cyanine3 maleimide is a thiol mono reactive dye.
Cy5-馬來酰亞胺酯 Cyanine5 maleimide
Cyanine5 maleimide reacts with thiols to give dye labeled conjugates.
Cy5.5-馬來酰亞胺酯 Cyanine5.5 maleimide
Cyanine5.5 maleimide is a thiol reactive dye for protein labeling.
Cy7-馬來酰亞胺酯 Cyanine7 maleimide
Cyanine7 maleimide is a sulfhydryl-reactive NIR dye.
Cy7.5-馬來酰亞胺酯 Cyanine7.5 maleimide
Cyanine7.5 maleimide is a thiol reactive near infrared dye.
磺酸基-Cy3-馬來酰亞胺酯 Sulfo-Cyanine3 maleimide
Water soluble thiol-reactive derivative of Sulfo-Cyanine3, a Cy3® analog
磺酸基-Cy5-馬來酰亞胺酯 Sulfo-Cyanine5 maleimide
Thiol reactive, water soluble cyanine dye for Cy5® channel.
水溶性近紅外熒光染料Sulfo-Cy7 maleimide
