人高密度脂蛋白（HDL）ELISA檢測(cè)試劑盒子科生物研發(fā)生產(chǎn)現(xiàn)貨供應(yīng)，深圳子科生物提供大量品牌進(jìn)口原裝，分裝，以及穩(wěn)定性強(qiáng)的自產(chǎn)國(guó)產(chǎn)人ELISA試劑盒，大鼠ELISA試劑盒，小鼠ELISA試劑盒，魚(yú)ELISA試劑盒，植物ELISA試劑盒，牛羊豬雞ELISA試劑盒性?xún)r(jià)比*。咨詢(xún)！
［產(chǎn)品名稱(chēng)］人高密度脂蛋白（HDL）ELISA檢測(cè)試劑盒
［英文名稱(chēng)］Human High density lipoprotein,HDL ELISA Kit
［貨號(hào)］ZK-H1128
［試劑盒］保存條件、保質(zhì)期2-8℃低溫保存下，6個(gè)月的保質(zhì)期。
［運(yùn)輸方式］當(dāng)?shù)乜蛻?hù)可以由專(zhuān)門(mén)配送人員免費(fèi)送貨上門(mén)貨快遞，外地客戶(hù)當(dāng)天下單當(dāng)天免費(fèi)快遞送貨上門(mén)。
［技術(shù)服務(wù)］凡購(gòu)買(mǎi)子科生物子科生物任何一款ELISA酶聯(lián)免疫分析檢測(cè)試劑盒，都可以享受免費(fèi)代測(cè)服務(wù)，外地客戶(hù)可以由技術(shù)老師的指導(dǎo)下正確放置好樣本的保存方式后快遞郵寄到我公司技術(shù)部，本地客戶(hù)可以享受免費(fèi)上門(mén)取樣服務(wù)！
人高密度脂蛋白（HDL）ELISA檢測(cè)試劑盒要想選對(duì)合適品牌的ELISA試劑盒，請(qǐng)您放心購(gòu)買(mǎi)我司ZIKER子科生物品牌試劑盒，高靈敏度、重復(fù)性，強(qiáng)特異性、操作性，值得您的選擇，讓您有一個(gè)滿(mǎn)意的實(shí)驗(yàn)結(jié)果！
ELISA試劑盒檢測(cè)方法：雙抗體夾心法、雙抗原夾心法、間接法測(cè)抗體、雙位點(diǎn)一步法、 競(jìng)爭(zhēng)法 、捕獲法、ABS-ELISA方法，我們子科生物主要采用雙抗體夾心法。詳細(xì)可以咨詢(xún)我們銷(xiāo)售人員。
Assay procedure
1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.
2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.
3. Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.
4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C.
5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.
6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.
7. Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not
appear uniform, gently tap the plate to ensure thorough mixing.
8. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.
Calculation of results
1.This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis.
2.First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software.
3.To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration.
4.Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.
5.The sensitivity by this assay is 10.0 pg/ml
6.Standard curve
Storage： 2-8℃.
validity： six months.
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