人可溶性晚期糖基化終末產物受體（sRAGE）ELISA檢測試劑盒

●英文名稱：Human sRAGE ELISA Kit

●產品規格：96T（人份）/48T（人份）
●單孔檢測：96T，可以檢測90份樣本，留5個孔做標準曲線，一個孔做空白對照；
          48T，可以檢測42份樣本，留5個孔做標準曲線，一個孔做空白對照；
●品牌：子科生物ZIKER,美國R&D,美國immonoway,美國sciencell,德國IBL
●適用范圍：實驗結果僅供科研參考，不推薦用于臨床診斷結果。

人可溶性晚期糖基化終末產物受體（sRAGE）ELISA檢測試劑盒操作步驟:
1.  從室溫平衡20min后的鋁箔袋中取出所需板條，剩余板條用自封袋密封放回4℃。
2.  設置標準品孔和樣本孔，標準品孔各加不同濃度的標準品50μL；
3.  樣本孔先加待測樣本10μL，再加樣本稀釋液40μL；空白孔不加。 
4.  除空白孔外，標準品孔和樣本孔中每孔加入辣根過氧化物酶（HRP）標記的檢測抗體100μL，用封板膜封住反應孔，37℃水浴鍋或恒溫箱溫育60min。
5.  棄去液體，吸水紙上拍干，每孔加滿洗滌液，靜置1min，甩去洗滌液，吸水紙上拍干，如此重復洗板5次（也可用洗板機洗板）。
6.  每孔加入底物A、B各50μL，37℃避光孵育15min。
7.  每孔加入終止液50μL，15min內，在450nm波長處測定各孔的OD值。

試劑盒性能:
1. 準確性：標準品線性回歸與預期濃度相關系數R值，大于等于0.9900。
2. 靈敏度：zui低檢測濃度小于1.0 pg/mL。
3. 特異性：不與其它可溶性結構類似物交叉反應。
4. 重復性：板內、板間變異系數均小于15%。
5. 貯藏：2-8℃，避光防潮保存。
6. 有效期：6個月

結果判斷:
繪制標準曲線：在Excel工作表中，以標準品濃度作橫坐標，對應OD值作縱坐標，繪制出標準品線性回歸曲線，按曲線方程計算各樣本濃度值。

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