［產品名稱］人水通道蛋白2（AQP-2）ELISA檢測試劑盒
［英文名稱］Human Aquaporin 2,AQP-2 ELISA Kit［貨號］ZK-H205［試劑盒］保存條件、保質期2-8℃低溫保存下，6個月的保質期。［運輸方式］當地客戶可以由專門配送人員免費送貨上門貨快遞，外地客戶當天下單當天免費快遞送貨上門。［技術服務］凡購買子科生物子科生物任何一款ELISA酶聯免疫分析檢測試劑盒，都可以享受免費代測服務，外地客戶可以由技術老師的指導下正確放置好樣本的保存方式后快遞郵寄到我公司技術部，本地客戶可以享受免費上門取樣服務！

深圳子科生物自主研發酶聯免疫分析檢測試劑盒，品牌為ZIKER,可以檢測Human，Rat、Mouse、Porcine、Sheep、Monkey、Goat、Bovine、Canine、Rabbit、Chicken、Duck、Fish、Plant  等各種樣本，品種齊全，質量保證，免費代測、技術全程支持服務，我公司長期跟各大高校科研實驗室保持課題合作，經驗豐富，質量穩定！我們所有的產品都是我司直接供貨，無中間差價，直接供貨，讓您以*惠的實驗預算完成您的實驗！

人水通道蛋白2（AQP-2）ELISA檢測試劑盒ELISA試劑盒檢測方法：雙抗體夾心法、雙抗原夾心法、間接法測抗體、雙位點一步法、 競爭法 、捕獲法、ABS-ELISA方法，我們子科生物主要采用雙抗體夾心法。詳細可以咨詢我們銷售人員。

Assay procedure
1.  Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.
2.  Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.
3.  Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.
4.  Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 
5.  Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.
6.  Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.
7.  Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not 
appear uniform, gently tap the plate to ensure thorough mixing.
8.  Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.
Calculation of results
1.This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 
2.First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. 
3.To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 
4.Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.
5.The sensitivity by this assay is 10.0 pg/ml
6.Standard curve
Storage：  2-8℃.
validity： six months.

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