roche公司貨號：11347101001   規格：25 mU

Endo-α-N-acetylgalactosaminidase
O-Glycan peptide hydrolase
O-Glycopeptide endo-D-galactosyl-N-acetyl-α-galactosamino hydrolase
 

Contents

Solution in 50 mM Na-phosphate, 0.02% Micr-O-protect, 350 mM NaCl, pH 7.5, stabilized with BSA [bovine serum albumin （68 kD）].

O-Glycosidase is used to release the Gal-β（1,3）-GaINAc unit from O-glycans （glycoproteins or glycopeptides）. Bindings to serine as well as to threonine are hydrolyzed. Substituents at the disaccharide （e.g., sialic acid） prevent the hydrolysis and, therefore, have to be removed chemically or enzymatically （e.g., with neuraminidase from Arthrobacter or fromVibrio cholerae）.

EC 3.2.1.97
Optimum pH: 6.0 - 7.6
Molecular weight: M_r = approximay 160 kD
Specific activity: approximay >2.5 U/mg enzyme protein at +37°C and pH 5.0 with 4-NP-GalNAc-Gal as the substrate
Specificity: Hydrolyzes Galβ1→ 3GalNAc from O-glycans
 

Principle

Gal-β（1,3）-GaINAc -↓- （Ser/Thr） -peptide/protein （O-Glycosidase）