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      CRL-9068 NCI-H929 人骨髓瘤細胞株

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      產品型號CRL-9068 NCI-H929

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      CRL-9068 NCI-H929 人骨髓瘤細胞株,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*培養條件!

      詳細介紹

      CRL-9068 NCI-H929 人骨髓瘤細胞株 的詳細介紹
      CRL-9068 NCI-H929 人骨髓瘤細胞株
      ATCC® Number: CRL-9068™    Price: $309.00
      Designations: NCI-H929 [H929]
      Depositors:  National Cancer Institute
      Isotype: IgA kappa (Recent tests for IgA, kappa have not detected the production of IgA; the cells are producing kappa light chain.)
      Biosafety Level: 1
      Shipped: frozen
      Medium & Serum: See Propagation
      Growth Properties: suspension
      Organism: Homo sapiens (human)
      Morphology: lymphoblast

      Source: Organ: bone marrow
      Disease: plasmacytoma; myeloma
      Cell Type: B lymphocyte;
      Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
       
      Receptors: transferrin
      Antigen Expression: PCA-1; CD38 +
      DNA Profile (STR): Amelogenin: X
      CSF1PO: 11
      D13S317: 12
      D16S539: 9,13
      D5S818: 11,12
      D7S820: 10,12
      THO1: 9.3
      TPOX: 8,11
      vWA: 14,15
      Cytogenetic Analysis: Near tetraploid. Most copies of chromosome 8 have the 8q+ abnormality.
      Age: 62 years
      Gender: female
      Ethnicity: Caucasian
      Comments: This cell line was established from a malignant effusion in a patient with myeloma.
      The cells are positive for plasma cell antigen 1 (PCA-1), transferrin receptor, CD38 (T10), but are negative for HLA DR, CALLA and markers of early B cell development.
      They are negative for Epstein-Barr virus nuclear antigen (EBNA).
      The cells have a rearrangement of the c-myc proto oncogene and express c-myc RNA.
      There is also an activated ras allele.
      Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
      Atmosphere: air, 95%; carbon dioxide (CO2), 5%
      Temperature: 37.0°C
      Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 4 X 10(5) viable cells/ml.
      Interval: Maintain cultures at cell concentrations between 5 X 10(5) and 1 X 10(6) viable cells/ml.
      Medium Renewal: 2 to 3 times per week
      Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
      Storage temperature: liquid nitrogen vapor phase
      Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
      recommended serum:ATCC 30-2020
      References: 22372: Hollis GF, et al. Complex translocation disrupts c-myc regulation in a human plasma cell myeloma. Mol. Cell. Biol. 8: 124-129, 1988. PubMed: 3275865
      23349: Gazdar AF, et al. Establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene. Blood 67: 1542-1549, 1986. PubMed: 2423157
      23351: Ernst TJ, et al. Identification of a second transforming gene, rasn, in a human multiple myeloma line with a rearranged c-myc allele. Blood 72: 1163-1167, 1988. PubMed: 3048435


      上海復祥生物提供 ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*培養條件,上有細胞照片,手機xiangfbio.詳情請登陸http://www.xiangbio.com/歡迎各位老師!
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      CRL-9068 NCI-H929 人骨髓瘤細胞株
      大鼠回腸細胞 IEC-18 CRL-1589
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      人胰腺癌細胞 Panc 02.03 CRL-2553
      人胰腺癌細胞 Panc 08.13 CRL-2551
      人胰腺癌細胞 Panc 03.27 CRL-2549
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      人成神經瘤細胞-骨髓 SK-N-DZ CRL-2149
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      人正常前列腺上皮細胞 RWPE-1 CRL-11609
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      人橫紋肌肉瘤細胞 A-204 HTB-82
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      人胰腺癌細胞 Capan-1 HTB-79
      人乳腺導管癌細胞 MDA-MB-435S HTB-129
      人胃癌細胞 Hs 746T HTB-135
      人肺腺癌細胞 NCI-H441 [H441] HTB-174
      人骨髓瘤細胞 U266  TIB-196
      人結腸癌細胞 NCI-H716 [H716] CCL-251
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      人腎透明細胞癌細胞 Caki-2 HTB-47
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      HTB-112 HEC-1-A 人子宮內膜腺癌細胞
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      BS-C-1 非洲綠猴腎細胞
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      Renca  小鼠腎癌細胞
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      P388小鼠白血病細胞
      opm-2 骨髓瘤細胞
      MDA-MB-435 人乳腺癌高轉移細胞
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      NIH:OVCAR-3 人卵巢癌細胞
      SW 1990人胰腺癌細胞系
      S180人結腸腺癌細胞
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      NCI-H446 人小細胞肺癌細胞
      MSTO-211H 人肺癌細胞
      H4-Ⅱe 大鼠肝癌細胞系
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      LS 180人結腸腺癌細胞
      HuTu-80十一指腸腺癌
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      REH人急性非B非T淋巴細胞白血病
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      RKO 人結腸腺癌細胞
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      RK-13 兔腎細胞系

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