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CRL-2557 Panc 05.04 人胰腺癌細(xì)胞，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和*培養(yǎng)條件！

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CRL-2557 Panc 05.04 人胰腺癌細(xì)胞 的詳細(xì)介紹

CRL-2557 Panc 05.04 人胰腺癌細(xì)胞

ATCC^® Number:

CRL-2557™

Price:

$417.00

Designations:	Panc 05.04
Depositors:	EM Jaffee
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens （human）
Morphology:	epithelial
Source:	Organ: pancreas Disease: adenocarcinoma
Cellular Products:	cytokeratins 7 and 18 [50655]
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation:	Isolation date: May 5, 1995
Tumorigenic:	Yes
Oncogene:	K-ras +
Antigen Expression:	MHC class I +; MHC class II - [50655] Blood type B; Rh+
Age:	77 years adult
Gender:	female
Ethnicity:	White
Comments:	Panc 05.04 is a pancreatic adenocarcinoma epithelial cell line derived, in 1995, from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma. The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655] The cells have a reported plating efficiency of *. [50655]
Propagation:	ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 20 Units/ml human recombinant insulin, 85%; fetal bovine serum, 15% Atmosphere: air, 95%; carbon dioxide （CO2）, 5% Temperature: 37.0°C
Subculturing:	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. Incubate cultures at 37°C. *Subction Ratio:** A subction ratio of 1:3 to 1:5 is recommended Medium Renewal:* Add media once per week. Fluid change one to two times per week.
Preservation:	Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO Storage temperature: liquid nitrogen vapor phase
Doubling Time:	46 hrs
Related Products:	Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001 recommended serum:ATCC 30-2020
References:	50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

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CRL-2557 Panc 05.04 人胰腺癌細(xì)胞
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大鼠回腸細(xì)胞 IEC-18 CRL-1589
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人正常前列腺成肌纖維細(xì)胞 WPMY-1 CRL-2854
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人肝癌細(xì)胞 SNU-398 CRL-2233
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人胰腺癌細(xì)胞 HPAC CRL-2119
人胰腺癌細(xì)胞 HPAF-II CRL-1997
人黑色素瘤細(xì)胞 A375.S2 CRL-1872
人臍靜脈內(nèi)皮細(xì)胞 HUV-EC-C CRL-1730
人黑素瘤細(xì)胞 WM-266-4 CRL-1676
人膀胱癌細(xì)胞 HT-1376 CRL-1472
人正常前列腺上皮細(xì)胞 RWPE-2 CRL-11610
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人胰腺癌細(xì)胞 Capan-2 HTB-80
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人肺腺癌細(xì)胞 NCI-H441 [H441] HTB-174
人骨髓瘤細(xì)胞 U266 TIB-196

人結(jié)腸癌細(xì)胞 NCI-H716 [H716] CCL-251

人多形性惡性膠質(zhì)瘤 T98G [T98-G] CRL-1690
人神經(jīng)母細(xì)胞瘤細(xì)胞 BE（2）-C CRL-2268
人腎透明細(xì)胞癌細(xì)胞 Caki-2 HTB-47
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CRL-5826 NCI-H226 人肺鱗癌細(xì)胞系
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U14 小鼠宮頸癌細(xì)胞
CRL-9518 L5178Y 小鼠淋巴瘤細(xì)胞
HTB-112 HEC-1-A 人子宮內(nèi)膜腺癌細(xì)胞
HTB-113 HEC-1-B 人子宮內(nèi)膜腺癌細(xì)胞
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ROS1728 大鼠成骨細(xì)胞
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CCL-127 IMR-32 人神經(jīng)母細(xì)胞瘤細(xì)胞
HTB-79 Capan-2 人胰腺癌細(xì)胞
BALL-1 人B淋巴細(xì)胞急性白血病細(xì)胞
CRL-2922 Eayh926 人臍靜脈細(xì)胞融合細(xì)胞
WB-F344 大鼠肝干細(xì)胞
CRL-1715 TM4 正常小鼠睪丸細(xì)胞
CRL-2539 4T1 小鼠乳腺癌細(xì)胞
CRL-1442 BRL-3A 大鼠肝細(xì)胞
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RSC96大鼠雪旺細(xì)胞
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HTB-113 hec-1-b 人子宮內(nèi)膜腺癌細(xì)胞
PLA-802?人惡性橫紋肌肉瘤
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opm-2 骨髓瘤細(xì)胞
MDA-MB-435 人乳腺癌高轉(zhuǎn)移細(xì)胞
KU812 人外周血嗜堿性白細(xì)胞
NIH:OVCAR-3 人卵巢癌細(xì)胞
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S180人結(jié)腸腺癌細(xì)胞
HuTu-80十二指腸腺癌
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NCI-H446 人小細(xì)胞肺癌細(xì)胞
MSTO-211H 人肺癌細(xì)胞
H4-Ⅱe 大鼠肝癌細(xì)胞系
HLF 人肺成纖維樣細(xì)胞
LS 180人結(jié)腸腺癌細(xì)胞
HuTu-80十一指腸腺癌
ST豬睪丸細(xì)胞系
REH人急性非B非T淋巴細(xì)胞白血病
NCI-H520 人肺鱗癌細(xì)胞
RKO 人結(jié)腸腺癌細(xì)胞
293A 人胚腎細(xì)胞
RK-13 兔腎細(xì)胞系

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