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HTB-79 Capan-1 人胰腺癌細胞

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HTB-79 Capan-1 人胰腺癌細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和*培養條件！

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HTB-79 Capan-1 人胰腺癌細胞 的詳細介紹

HTB-79 Capan-1 人胰腺癌細胞

ATCC^® Number:

HTB-79™

Price:

$269.00

Designations:	Capan-1
Depositors:	J Fogh
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens （human）
Morphology:	epithelial
Source:	Organ: pancreas Disease: adenocarcinoma Derived from metastatic site: liver
Cellular Products:	mucin
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions:	The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.） The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.） Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone （212） 639-6181; （212） 717-3439.
Applications:	transfection host （Roche FuGENE® Transfection Reagents technology from amaxa）
Receptors:	progesterone receptor, positive [34271] estrogen receptor, alpha, positive [34271]
Tumorigenic:	Yes
Antigen Expression:	Blood Type A; Rh+; HLA A2, A9, B13, B17
DNA Profile （STR）:	Amelogenin: X CSF1PO: 11 D13S317: 9 D16S539: 13,14 D5S818: 11 D7S820: 10,11 THO1: 6 TPOX: 8,11 vWA: 16
Cytogenetic Analysis:	（P7） hypotriploid with abnormalities including dicentrics, breaks, acrocentric fragments, large submetacentric and subocentric chromosomes plus minute marker
Isoenzymes:	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1-2 PGM3, 1
Age:	40 years
Gender:	male
Ethnicity:	Caucasian
Comments:	The cells will slough off of the growth surface if they become too heavy. Capan-1 expresses the cystic fibrosis transmembrane conductance regulator （CFTR） and secrete gastric type mucins.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Temperature: 37.0°C
Subculturing:	*Subction Ratio:** A subction ratio of 1:2 to 1:4 is recommended Medium Renewal:* Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation:	culture medium 95%; DMSO, 5%
Related Products:	Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2005 recommended serum:ATCC 30-2020
References:	22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 22986: Fanjul M, Hollande E. Morphogenesis of "duct-like" structures in three-dimensional cultures of human cancerous pancreatic duct cells （Capan-1）. In Vitro Cell. Dev. Biol. Anim. 29A: 574-584, 1993. PubMed: 8354666 23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903 23153: Chambers JA, Harris A. Expression of the cystic fibrosis gene and the major pancreatic mucin gene, MUC1, in human ductal epithelial cells. J. Cell Sci. 105: 417-422, 1993. PubMed: 7691840 23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 34271: Hollande E, et al. Expression of estrogen receptors during growth of human pancreatic adenocarcinoma cells （Capan-1）-relationship with differentiation. In Vitro Cell. Dev. Biol. Anim. 34: 593-599, 1998. PubMed: 9719420

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HTB-79 Capan-1 人胰腺癌細胞
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CRL-1715 TM4 正常小鼠睪丸細胞
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MSTO-211H 人肺癌細胞
H4-Ⅱe 大鼠肝癌細胞系
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293A 人胚腎細胞
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