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CRL-10317 MCF-10A 人正常乳腺上皮細胞 的詳細介紹

CRL-10317 MCF-10A 人正常乳腺上皮細胞

ATCC®Number:CRL-10317™ Price:$272.00
Designations:MCF 10ADepositors: Michigan Cancer FoundationBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens（human）Morphology:epithelial

Source:Organ:mammary gland; breast
Disease:fibrocystic disease
Cell Type:epithelialPermits/Forms:In addition to theMTAmentioned above, otherATCC and/or regulatory permitsmay be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Pleaseclick herefor information regarding the specific requirements for shipment to your location. Isolation:Isolation date:August 22, 1984Applications:transfection host （Roche FuGENE® Transfection Reagents）Tumorigenic:NoDNA Profile （STR）:Amelogenin: X
CSF1PO: 10,12
D13S317: 8,9
D16S539: 11,12
D5S818: 10,13
D7S820: 10,11
THO1: 8,9.3
TPOX: 9,11
vWA: 15,17Isoenzymes:AK-1, 1 [23084]
ES-D, 1 [23084]
G6PD, B [23084]
GLO-I, 1-2 [23084]
PGM1, 1-2 [23084]
PGM3, 1 [23084]Age:36 yearsGender:femaleEthnicity:CaucasianComments:The MCF 10A cell line is a non-tumorigenic epithelial cell line. [21968]
The line was produced by long term culture in serum free medium with low Ca++ concentration. [21968]
MCF 10A was derived from adherent cells in the population. [21968]
Cells derived from a floating population are available （see MCF 10F, ATCC CRL-10318）. [21968]
The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen. [21968]
They exhibit three dimensional growth in collagen, and form domes in confluent cultures. [21968]
Thus far, the cells have shown no signs of terminal differentiation or senescence.
The line is responsive to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor （EGF）. [21968]
By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells. [23085]
They also express breast specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies. [23085]
The calcium content of the medium exerts a strong effect on the morphology of the cells. [22248]Propagation:ATCC complete growth medium:The base medium for this cell line （MEBM） along with the additives can be obtained from Lonza/Clonetics Corporation as a kit: MEGM, Kit Catalog No. CC-3150. ATCC does not use the GA-1000 （gentamycin-amphotericin B mix） provided with the BEGM kit. To make the complete growth medium, you will need to add the following components to the kit （sold separay）:
100 ng/ml cholera toxin
Note: Do not filter complete medium
Temperature:37.0°C
Atmosphere:air, 95%; carbon dioxide （CO2）, 5%Subculturing:Protocol:Remove medium and rinse monolayer with PBS （ATCC Cat# 30-2200）.Add 3.0 ml 0.05% trypsin, 0.53 mM EDTA and incubate at 37C for 15 minutes. To neutralize trypsin, add 3 ml solution of 0.1% soybean trypsin inhibitor.Centrifuge cell suspension at 125 xg for 5 to 10 minutes.Resuspend cell pellet in complete culture medium. Add appropriate aliquots of cell suspension to new culture vessels.
Subc*tion Ratio:A subc*tion ratio of 1:3 to 1:4 is recommended
Medium Renewal:Every 2 to 3 daysPreservation:Freeze medium:Complete growth medium supplemented with 7.5% （v

） DMSO
Storage temperature:liquid nitrogen vapor phaseRelated Products:derived from same individual:ATCCCRL-10318
derived from same individual:ATCCCRL-10780
derived from same individual:ATCCCRL-10781References:

21968: Soule H, McGrath CM. Immortal human mammary epithelial cell lines. US Patent 5,026,637 dated Jun 25 1991

22025: Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,206,165 dated Apr 27 1993

22248: Soule HD, McGrath CM. A simplified method for passage and long-term growth of human mammary epithelial cells. In Vitro Cell. Dev. Biol. 22: 6-12, 1986. PubMed:2418007

23084: Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed:1975513

23085: Tait L, et al. Ultrastructural and immunocytochemical characterization of an immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6087-6094, 1990. PubMed:1697506

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CRL-10317 MCF-10A 人正常乳腺上皮細胞
大鼠回腸細胞 IEC-18 CRL-1589
非洲綠猴腎細胞 VERO C1008 [Vero 76, clone E6, Vero E6] CRL-1586
人成纖維細胞 Hs 738.St/Int CRL-7869
人正常前列腺上皮細胞 WPE-int CRL-2888
人正常前列腺成肌纖維細胞 WPMY-1 CRL-2854
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人胰腺癌細胞 Panc 05.04 CRL-2557
人胰腺癌細胞 Panc 04.03 CRL-2555
人胰腺癌細胞 Panc 02.03 CRL-2553
人胰腺癌細胞 Panc 08.13 CRL-2551
人胰腺癌細胞 Panc 03.27 CRL-2549
人胰腺癌細胞 Panc 10.05 CRL-2547
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人成神經瘤細胞-骨髓 SK-N-DZ CRL-2149
人胰腺癌細胞 HPAC CRL-2119
人胰腺癌細胞 HPAF-II CRL-1997
人黑色素瘤細胞 A375.S2 CRL-1872
人臍靜脈內皮細胞 HUV-EC-C CRL-1730
人黑素瘤細胞 WM-266-4 CRL-1676
人膀胱癌細胞 HT-1376 CRL-1472
人正常前列腺上皮細胞 RWPE-2 CRL-11610
人正常前列腺上皮細胞 RWPE-1 CRL-11609
雞胚胎成纖維細胞 UMNSAH/DF-1 CRL-12203
人乳腺癌細胞 MDA-MB-231 HTB-26
小鼠巨噬細胞 RAW 264.7 TIB-71
人橫紋肌肉瘤細胞 A-204 HTB-82

人胰腺癌細胞 Capan-2 HTB-80
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人胃癌細胞 Hs 746T HTB-135
人肺腺癌細胞 NCI-H441 [H441] HTB-174
人骨髓瘤細胞 U266 TIB-196
人結腸癌細胞 NCI-H716 [H716] CCL-251
人多形性惡性膠質瘤 T98G [T98-G] CRL-1690
人神經母細胞瘤細胞 BE（2）-C CRL-2268
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人皮膚成纖維細胞 Hs 865.Sk CRL-7601
CA46 人淋巴瘤細胞系
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Tca-8113 人舌鱗癌細胞
MA-891 小鼠乳腺癌高轉移細胞
U14 小鼠宮頸癌細胞
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HTB-112 HEC-1-A 人子宮內膜腺癌細胞
HTB-113 HEC-1-B 人子宮內膜腺癌細胞
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BALL-1 人B淋巴細胞急性白血病細胞
CRL-2922 Eayh926 人臍靜脈細胞融合細胞
WB-F344 大鼠肝干細胞
CRL-1715 TM4 正常小鼠睪丸細胞
CRL-2539 4T1 小鼠乳腺癌細胞
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BRL 大鼠肝細胞
RSC96大鼠雪旺細胞
Isk 人子宮內膜癌細胞
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PLA-802?人惡性橫紋肌肉瘤
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BS-C-1 非洲綠猴腎細胞
A498人腎癌細胞
3AO人卵巢癌細胞
iec-6 大鼠小腸隱窩上皮細胞
u87MG 人腦星形膠質母細胞瘤
Renca 小鼠腎癌細胞
nci-h929 骨髓瘤細胞
P388小鼠白血病細胞
opm-2 骨髓瘤細胞
MDA-MB-435 人乳腺癌高轉移細胞
KU812 人外周血嗜堿性白細胞
NIH:OVCAR-3 人卵巢癌細胞
SW 1990人胰腺癌細胞系
S180人結腸腺癌細胞
HuTu-80十二指腸腺癌

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MSTO-211H 人肺癌細胞
H4-Ⅱe 大鼠肝癌細胞系
HLF 人肺成纖維樣細胞
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