5GPepsin 1:10000 *1:10000 試劑
【簡單介紹】
【詳細說明】
試劑:Pepsin 1:10000 *1:10000 試劑
Pepsin 1:10000 *1:10000 試劑
編號 規格 價格 WK0499 5G 160.00
產品詳細說明:
Pepsin from porcine gastric mucosa
Synonym : Pepsin A
Pepsin from hog stomach
CAS Number : 9001-75-6
(EC) Number: 3.4.23.1
Descriptions:
Analysis Note: E1%/280=14.7
Protein determined by E1%/280
Application: Used to produce F(ab ′ )2 fragments of antibodies.
Biochem/physiol Actions: Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at
Val, Ala or Gly. Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine
HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH>6.
Unit Definition: One unit will produce a ΔA 280 of 0.001 per min at pH2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16ml. Light path = 1cm.)
Properties:
form ………………………………………………………………….powder
mol wt……………………………………………………………….mol wt 35 kDa
Gene Information ………………………………………………pig ... LOC396892(396892)
Risk Statements: 36/37/38-42
Safety Statements: 22-24-26-36/37
TEST | SPECIFICATION |
APPEARANCE | REPORT RESULT |
SOLUBILITY | CLEAR TO SLIGHTLY HAZY COLORLESS SOLUTION AT 1 MG/ML IN 0.01N HCL |
PROTEIN BY UV ABSORBANCE | REPORT RESULT |
LOSS ON DRYING | NMT 5% |
ENZYMATIC ACTIVITY | 800 TO 2500 UNITS/MG PROTEIN |
UNIT DEFINITION | ONE UNIT WILL PRODUCE A CHANGE IN A280 OF 0.001 PER MINUTE AT PH2.0 AT 37DEGC, MEASURED AS TCA-SOLUBLE PRODUCTS USING HEMOGLOBIN AS SUBSTRATE. (FINAL VOLUME = 16ML. LIGHTPATH=1CM.) |
RECOMMENDED RETEST | 2 YEARS |
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