牛LKX-2/CD2 ELISA試劑盒 說(shuō)明書(shū):
實(shí)驗(yàn)原理:
本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中(產(chǎn)品名稱)水平。用純化的其抗體包被微孔板,制成固相抗體。往包被單抗的微孔中依次加入本產(chǎn)品,再與HRP標(biāo)記的其抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過(guò)*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(OD值),通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中產(chǎn)品的濃度。
產(chǎn)品免費(fèi)代測(cè):牛LKX-2/CD2 ELISA試劑盒
英文名稱:Bovine lymphocyte function associated antigen 2,LKX-2 ELISA KIT
規(guī)格:96T/48T,定量/定性
保存條件:2-8℃,6個(gè)月
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