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Pierce 26146-Pierce Classic IP Kit原裝現(xiàn)貨

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  • 上海葉舟生物科技有限公司
  • 2022-06-20 14:55:24
  • 上海市
  • Pierce
  • 1130

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【簡(jiǎn)單介紹】

產(chǎn)品名稱:Pierce Classic IP Kit
貨號(hào):Pierce 26146
規(guī)格:KIT

【詳細(xì)說(shuō)明】

產(chǎn)品名稱:Pierce Classic IP Kit
貨號(hào):Pierce 26146
規(guī)格:KIT
The Thermo Scientific Pierce Classic IP Kit provides all the necessary reagents, spin cups and collection tubes to perform successful immunoprecipitation (IP) experiments with ease.
The kit uses high-capacity Protein A/G agarose affinity resin for efficient binding of most species and subclasses of IP antibodies. The included IgG elution buffer provides milder and less denaturing recovery of antibody:antigen complexes than the traditional method of boiling in reducing sample buffer for SDS-PAGE, facilitating a greater variety of methods for subsequent analysis. The microcentrifuge spin column format helps to ensure effective washing and separation of samples from the beaded agarose affinity resin.
Highlights:
Assay consistency – Pierce Spin Columns (see dimensions) eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes
Fast – Immunoprecipitate (IP) in less than one hour
Mild elution conditions – Recover antigen without harsh detergents or reducing agents
Easy-to-follow instructions – IP target protein from crude lysate in four simple steps
Suitable for most common antibody types – Protein A/G provides excellent binding for most mouse, rabbit, human and goat IgG subclasses
Complete kit – includes lysis/wash buffer, binding and elution buffers, control agarose resin, sample loading buffer and spin columns
Applications:
Routine, traditional immunoprecipitation experiments
Immunoprecipitation for analysis in nonreducing conditions
Product Details:
Like traditional IP methods, the Pierce Classic IP Kit procedure involves formation of antibody:antigen complexes in a sample solution and then capture of that complex to an IgG-binding protein that is covalently bound to beaded agarose resin (Protein A/G Agarose). After washing to remove nonbound (presumably undesired) components of the sample, the antigen and antibody are recovered from the beaded resin with elution buffer supplied in the kit. The entire procedure is performed in a microcentrifuge spin column, allowing solutions to be fully separated from the agarose resin upon brief centrifugation.

名稱 廠商 貨號(hào) 規(guī)格 目錄價(jià)
Pierce Classic IP Kit Pierce 26146 KIT 3518
Pierce Crosslink IP Kit Pierce 26147 KIT 4151
Pierce Direct IP Kit Pierce 26148 KIT 3518
Co-Immunoprecipitation Kit Pierce 26149 KIT 2718
BupH Tris Buffered Saline Packs Pierce 28376 40PS/PKG 1829
Disposable Polypropylene Columns, 1-5 ml Pierce 29922 100/PACK 2364
Disposable Polypropylene Columns, 2-10 ml/(一次性的聚丙烯柱 Pierce 29924 100/PKG 2589
NeutrAvidin Protein Pierce 31000 10 MG 1664
Activated Peroxidase Antibody Labeling Kit Pierce 31497 KIT 5746
Fluoro-Goat Anti-Mouse IgG (H+L) Pierce 31569 2 MG 1604
Goat Anti-Human IgG [F(ab´)]Texas Red Conjugated Pierce 31945 2 MG 詢價(jià) 
Pierce ECL Western Blotting Substrate/免疫印跡化學(xué)發(fā)光試劑 Pierce 32106 500 ML  1975

    
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