Description
Application SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem （Pharmacia） and Schagger and von Jagow SDS-PAGE （Tricine）. SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximay equal intensity after electrophoresis and Coomassie blue staining.
Other Notes Protein （mol. wt.）
Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle （36,000）
Ovalbumin, chicken egg （45,000）
Glutamic dehydrogenase, bovine liver （55,000）
Albumin, bovine serum （66,000）
Phosphorylase B, rabbit muscle （97,000）
β-Galactosidase, E. coli （116,000）
Myosin, porcine heart （200,000）
Will yield bands for the following seven proteins from the SigmaMarker list with Coomassie Blue staining.
Reconstitution Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 0.5% dithiothreitol, 2% SDS, and 0.0005% bromophenol blue.
Legal Information SigmaMarker is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
Properties
form lyophilized powder
usage sufficient for 10-20 large gels （16 × 18 cm）
sufficient for 20-30 mini-gels （10 × 10 cm）
vial sufficient for 50-100 PhastGels
mol wt mol wt 36,000-200,000 Da
storage temp. 2-8°C
Safety
Personal Protective Equipment Eyeshields, Gloves, type N95 （US）, type P1 （EN143） respirator filter
WGK Germany 3
References
reference Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685, （1970） Abstract
Schagger, H. and von Jagow, G., Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368-379, （1987）
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