Description
Application Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Biochem/physiol Actions Lysozyme hydrolyzes β（1→4） linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
The enzyme is active over a broad pH range （6.0 to 9.0）. At pH 6.2, maximal activity is observed over a wider range of ionic strengths （0.02 to 0.100 M） than at pH 9.2 （0.01 to 0.06 M）.
Other Notes Suitable for the hydrolysis of bacterial cell walls, mucopolysaccharides, mucopolypeptides or chitin; degradability of L. lactis subsp. cremoris H2 cell walls1; Review2,3,4
Unit Definition 1 U corresponds to the amount of enzyme which decreases the absorbance at 450 nm by 0.001 per minute at pH 7.0 and 25°C （Micrococcus luteus, ATCC 4698, as substrate）
Properties
form powder
quality dialyzed
lyophilized
mol wt single-chain mol wt 14.3 kDa
Mr ~14600
storage temp. 2-8°C
Safety
Personal Protective Equipment Eyeshields, Gloves, type N95 （US）, type P1 （EN143） respirator filter
Safety Statements 22-24/25
WGK Germany 3
RTECS OL5989000
References
Cited Reference 1. T. Coolbear et al. Appl. Environ. Microbiol. 58, 3263, （1992） Abstract
2. P. Jolles, J. Jolles Mol. Cell Biochem. 63, 165, （1984） Abstract
3. L. Stevens Comp. Biochem. Physiol., B: Comp. Biochem. 100B, 1, （1991）
4. R.W. Franck Bioorg. Chem. 20, 77, （1992）
reference Jolles, P. Angew. Chem. Int. Ed. Engl. 8, 227-239, （1969）
Rupley, J.A. Biochim. Biophys. Acta 83, 245-255, （1964）
Holler, H., et al. Biochemistry 14, 2377-2385, （1975）
Canfield, R.E. J. Biol. Chem. 238, 2698-2707, （1963）
Davies, R.C., et al. Biochim. Biophys. Acta 178, 294-305, （1969）
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MSDS信息
提供商 語言
Lysozyme 英文
SigmaAldrich 英文
溶菌酶 用途與合成方法
化學性質 白色或微白色凍干粉，溶于水，不溶于乙醚和丙酮，pI為11.0-11.35，zui適pH值6.5。穩定性：酸性介質中可穩定存在，堿性介質中易失活；96℃， pH值為3條件下，15min后活力保持87%。抑制劑有碘、咪唑和吲哚衍生物、表面活性劑（十二烷基硫酸鈉、醇類和碳鏈不少于12的脂肪酸）。1%水溶液在281.5nm處的吸光系數為26.4。通過水解細菌細胞壁的肽聚糖來溶菌，酶反應： Micrococcus luteus或Micrococcus lysodeikticus菌懸浮液═溶菌并澄清。
用途 用于生化研究，臨床上用于急慢性咽喉炎、扁平苔癬、扁平疣等疾病的治療。
生產方法 以蛋清為原料，在pH6.5條件下用弱酸性陽離子交換樹脂732吸附后，再用硫酸銨洗脫，經透析后冷凍干燥得產品。
