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北京瑞達恒輝科技發展有限公司
北京瑞達恒輝科技發展有限公司
CAS號： 產品編號：A19654 品牌 : CAS號： 別名: ......
背景：
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]
| 免疫原 | A synthesized peptide derived from human active + pro Caspase-3. |
|---|---|
| 序列 | for sequence |
| 基因ID | 836 |
| Swiss Prot | P42574 |
| 別名 | CPP32;CPP32B;SCA-1;Active Caspase 3;CASP3;active Caspase-3;Caspase 3;Caspase-3 p12;caspase-3 |
| 反應物種 | Human, Mouse, Rat |
|---|---|
| 驗證應用 | WBIF |
| 用戶驗證的應用 | WB (Sanghuangporus vaninii , Homo sapiens , Giardia duodenalis , Mus musculus) |
| 推薦稀釋比&實驗步驟 |
|
| 理論分子量 | 17kDa/32kDa |
| 實際分子量 | 17KDa/32kDa/35KDa |
| 存儲緩沖液 | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| 實驗方法 | Western blotting |
| 陽性樣本 | Jurkat,Mouse lung,Mouse liver,NIH/3T3,Rat spleen,Rat liver,293T |
| 細胞定位 | Cytoplasm |
| 純化方式 | Affinity purification |
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