當前位置：賽默飛世爾科技（中國）有限公司>>蛋白分析試劑>>蛋白純化>>Bac-to-Bac™ HBM TOPO™ 分泌表達系統

Bac-to-Bac™ HBM TOPO™ 分泌表達系統

返回列表頁

參考價

面議

具體成交價以合同協議為準

產品型號Gibco

品牌

廠商性質其他

所在地

在線詢價收藏產品查看聯系電話

聯系方式：查看聯系方式

更新時間：2023-11-06 17:41:47瀏覽次數：161次

聯系我時，請告知來自環保在線

產品分類 品牌分類

  蛋白分析試劑

蛋白質譜檢材制備

親和素-結合產品

蛋白檢測

透析及脫鹽

蛋白純化

蛋白檢測系統

蛋白樹脂

蛋白提取

生物活性小分子

氨基酸分析試劑和附件

抗體親和純化緩沖液

感受態細胞

基因報告系統

免疫組織化學試劑盒和試劑

親和素產品

SILAC 同位素標記試劑和試劑盒

全部產品列表

暫無信息

賽默飛世爾科技（中國）有限公司

經營模式：其他

商鋪產品：2514條

所在地區：

詢價 給他留言

產品簡介

Bac-to-Bac™ HBM TOPO™ 分泌表達系統

詳細介紹

Bac-to-Bac baculovirus expression is an efficient method for producing baculovirus for high-level protein expression in insect cells. It relies on generation of recombinant virus by site-specific transposition in E. coli rather than homologous recombination in insect cells. The pFastBac HBM TOPO expression system features:

? Expression of secreted proteins—The Bac-to-Bac HBM TOPO vector contains the honeybee melittin (HBM) secretion signal to enable secreted protein expression. Because glycoproteins cannot be glycosylated in the absence of a secretion signal, this vector is recommended for the expression of glycoproteins.

? Flexibility—The Bac-to-Bac HBM TOPO vector contains a C-terminal His-tag with a TEV cleavage site to enable easy purification of His-tagged proteins with nickel-chelating resins (such as the Invitrogen ProBond Purification System) and generation of native proteins with the aid of the Invitrogen AcTEV Protease.

? Fast cloning, easy screening—Blunt TOPO cloning technology enables cloning of blunt-end PCR products into the pFastBac TOPO vector in five minutes. In addition, the pFastBac TOPO expression kit is supplied with Mach1-T1R E. coli that enable the visualization of colonies eight hours after plating on ampicillin-selective plates due to their faster doubling time compared to other standard cloning strains. Find out more about the advantages of TOPO cloning ›

? High transfection efficiency with ExpiFectamine Sf Transfection Reagent—The Bac-to-Bac TOPO expression kits now come with the next-generation ExpiFectamine Sf Transfection Reagent for efficient DNA transfection in insect cells using fast, flexible protocols. Find out more about this reagent ›

? Time-saving expression bacmid—With the Bac-to-Bac system, the expression cassette of the pFastBac vector recombines with the parent bacmid in DH10Bac E. coli Competent Cells to form an expression bacmid. The bacmid is then transfected into insect cells for production of recombinant baculovirus particles.

? Easy colony screening—The parent bacmid in DH10Bac E. coli contains a segment of the lacZa gene. The lacZa gene is disrupted upon transposition of the expression cassette into the bacmid, allowing for blue/white selection of recombinants for easier identification of recombinant colonies.

? High protein expression—The pFastBac vector uses the strong polyhedrin promoter to generate high levels of expression in a variety of insect cell line such as Sf9, Sf21, and High Five cells.

For Research Use Only. Not for use in diagnostic procedures.