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ACQUITY UPLC BEH HILIC柱

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極性分析物的保留、HILIC

詳細介紹

ACQUITY UPLC BEH HILIC柱使用未鍵合BEH顆粒以提高對高極性分析物的保留能力，同時對于極性和可離子化物質的混合物提供相對于反相色譜的正交性的分離選擇性。該色譜柱經優化并測試，以確保在UPLC HILIC條件下能提供高效、重現的分離效果。該產品在設計伊始就針對克服既有HILIC固定相的一個重要弱點：化學穩定性。結實的BEH雜化顆粒的寬pH耐受能力拓寬了HILIC應用條件范圍，并顯著延長了柱使用壽命。

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毛茛黃酮類成分的UPLC HILIC/Q-TOF-MS分析

目的:建立超高效親水性液相色譜與串聯四級桿飛行時間質譜儀聯用技術(UPLC HILIC/Q-TOF-MS)對毛茛黃酮類成分進行分析的方法。方法:用ACQUITY UPLC BEH HILIC色譜柱,以甲醇∶水=1∶1含10mM乙酸銨(A)/乙腈∶甲醇=95∶5含10mM乙酸銨(B)梯度洗脫,使用ESI離子源,正、負離子模式下采集數據。結果:根據元素組成分析、二級質譜和相關文獻,鑒定、推斷出毛茛中11個主要成分,分別是小麥黃素,牡荊素,葒草素,異葒草素,芹菜素-6-C-β-D-葡萄糖苷-8-C-α-L-阿拉伯糖苷,小麥黃素-7-O-β-D-葡萄糖苷,蘆丁等。結論:經過超高效親水性液相色譜的分離,借助Q-TOF-MS測定的準確分子量及二級質譜信息可以判定毛茛中的主要黃酮類成分,為毛茛黃酮類成分分析提供了一種快速有效的方法。

品名	編號
ACQUITY - XBridge BEH HILIC Method Transfer Kit, 130?, 1.7 - 5 µm, 2.1 - 4.6 mm X 50 - 150mm, 2/p...	186004960
ACQUITY - XBridge BEH HILIC Method Transfer Kit, 130?, 1.7 - 3.5 µm, 2.1 - 4.6 mm X 100 - 150mm, ...	186004973
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 2.1 mm X 100 mm, 3/pkg	176001093
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 2.1 mm X 50 mm, 3/pkg	176001092
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 2.1 mm X 150 mm, 3/pkg	176001094
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 1 mm X 150 mm, 1/pkg	186003459
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 1 mm X 150 mm, 3/pkg	176001091
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 1 mm X 100 mm, 3/pkg	176001090
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 1 mm X 100 mm, 1/pkg	186003458
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 1 mm X 50 mm, 1/pkg	186003457
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 2.1 mm X 100 mm, 1/pkg	186003461
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 2.1 mm X 50 mm, 1/pkg	186003460
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 1 mm X 50 mm, 3/pkg	176001089
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 2.1 mm X 150 mm, 1/pkg	186003462
ACQUITY UPLC BEH HILIC Method Validation Kit, 130?, 1.7 µm, 2.1 mm X 100 mm, 3/pkg	186004054
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 3 mm X 150 mm, 3/pkg	176001812
ACQUITY UPLC BEH HILIC VanGuard Pre-column, 130?, 1.7 µm, 2.1 mm X 5 mm, 3/pkg	186003980
ACQUITY UPLC BEH HILIC Method Validation Kit, 130?, 1.7 µm, 2.1 mm X 50 mm, 3/pkg	186004053
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 2.1 mm X 75 mm, 1/pkg	186005608
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 3 mm X 50 mm, 3/pkg	176001810
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 3 mm X 100 mm, 3/pkg	176001811
ACQUITY UPLC BEH HILIC Method Validation Kit, 130?, 1.7 µm, 2.1 mm X 150 mm, 3/pkg	186006023
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 3 mm X 150 mm, 1/pkg	186004677
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 3 mm X 100 mm, 1/pkg	186004676
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 3 mm X 75 mm, 1/pkg	186005613
ACQUITY UPLC BEH HILIC Method Validation Kit, 130?, 1.7 µm, 3 mm X 50 mm, 3/pkg	186004699
ACQUITY UPLC BEH HILIC Method Validation Kit, 130?, 1.7 µm, 3 mm X 100 mm, 3/pkg	186004700
ACQUITY UPLC BEH HILIC Column, 130?, 1.7 µm, 3 mm X 50 mm, 1/pkg	186004675