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水樣調(diào)制葉綠素?zé)晒鈨x——WATER-PAM

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水樣調(diào)制葉綠素?zé)晒鈨x——WATER-PAM

詳細(xì)介紹

主要功能

  • 可測(cè)熒光誘導(dǎo)曲線并進(jìn)行淬滅分析

  • 可測(cè)光響應(yīng)曲線和快速光曲線(RLC)

  • 可測(cè)量水樣的下列光合指標(biāo)活性:

  •        光合效率和光合速率(相對(duì)電子傳遞速率)

  •        藻類(lèi)的潛在光合效率(“生長(zhǎng)潛能")

  •        藻類(lèi)的光保護(hù)能力

  • 藻類(lèi)耐受強(qiáng)光的能力

  • 51 個(gè)內(nèi)置模式菜單,方便參數(shù)設(shè)置和標(biāo)準(zhǔn)測(cè)量

  • 系統(tǒng) I 用于浮游植物研究,系統(tǒng) II 用于大型藻類(lèi)研究,系統(tǒng) III 用于連續(xù)監(jiān)測(cè)水體光合作用

 

測(cè)量參數(shù)

Fo, Fm, Fv/Fm, F, Fm’, Fo’, Y(II) 即 ΔF/Fm’, qP, qN, NPQ, ETR 和 PAR 等

 

應(yīng)用領(lǐng)域

測(cè)量野外自然水樣或?qū)嶒?yàn)室培養(yǎng)的微藻樣品的光合作用,三套系統(tǒng)可供選擇,可應(yīng)用于水生生物學(xué)、水域生態(tài)學(xué)、海洋學(xué)、湖沼學(xué)等領(lǐng)域,檢測(cè)限達(dá) 0.1 μgChl/L。可用于有害藻華的早期預(yù)警。與 PHYTO-PAM 的區(qū)別在于,WATER-PAM 不能進(jìn)行浮游植物分類(lèi)。

 

主要技術(shù)參數(shù)

  • 測(cè)量光:3 個(gè)波長(zhǎng)為 650 nm 的 LED 陣列。

  • 光化光:12 個(gè)波長(zhǎng)為 660 nm 的 LED 陣列,連續(xù)光強(qiáng) 2000 μmol m-2 s-1

  • 飽和脈沖:12 個(gè)波長(zhǎng)為 660 nm 的 LED 陣列,閃光強(qiáng)度 4000 μmol m-2 s-1

  • 遠(yuǎn)紅光源:3 個(gè)波長(zhǎng)為 740 nm 的 LED 陣列。

  • 信號(hào)檢測(cè):光電倍增管檢測(cè)器(H6779-01,Hamamatsu),過(guò)載保護(hù)功能,檢測(cè)信號(hào) λ > 710 nm。

  • 數(shù)據(jù)存儲(chǔ):CMOS RAM 128 KB,可存儲(chǔ)4000組數(shù)據(jù)。

 

選購(gòu)指南

Water-PAM-1.jpg

系統(tǒng) I(主機(jī) PAM-Control 和激發(fā)-檢測(cè)單元 WATER-ED),

適合現(xiàn)場(chǎng)采水樣測(cè)量或室內(nèi)微藻測(cè)量
       
WATER-EDF-0060.jpg-1.jpgWATER-PAM-02.jpg

系統(tǒng)II 激發(fā)-檢測(cè)單元 WATER-EDF1.5,

適合大型海藻、附著藻類(lèi)、水生植物等的測(cè)量

系統(tǒng)III 激發(fā)-檢測(cè)單元 WATER-FT,

適合隨船走航測(cè)量

  

三套系統(tǒng)的組成比較

系統(tǒng)組成
系統(tǒng)I
浮游植物版
系統(tǒng)II
附著藻類(lèi)/大型藻類(lèi)版
系統(tǒng)III
連續(xù)監(jiān)測(cè)版
野外現(xiàn)場(chǎng)或室內(nèi)測(cè)量,樣品為水樣或藻液野外現(xiàn)場(chǎng)或室內(nèi)測(cè)量,樣品為大型海藻、附著藻類(lèi)、水生植物、苔蘚、地衣、植物葉片等 
野外現(xiàn)場(chǎng)或室內(nèi)測(cè)量,需要抽水上來(lái),連續(xù)監(jiān)測(cè)光合活性
可選附件 1:攪拌器,對(duì)于易發(fā)生沉降或上浮的樣品非常重要2.jpg
可選附件 1:平面狀微型光量子探頭,用于測(cè)量樣品接收到的光強(qiáng)1.jpg 

可選附件 2:球狀微型光量子探頭,可放入系統(tǒng) I 的樣品杯中測(cè)量 PAR,也可放入水面下測(cè)量水體光場(chǎng)3.jpg
可選附件 2:手持式多功能 PAR 輻射儀,用于連接平面狀微型光量子探頭后,進(jìn)行獨(dú)立的光強(qiáng)測(cè)量4.jpg 

可選附件 3:手持式多功能 PAR 輻射儀,用于連接球狀微型光量子探頭后,進(jìn)行獨(dú)立的光強(qiáng)測(cè)量4.jpg




 

產(chǎn)地:德國(guó) WALZ

 

參考文獻(xiàn)

數(shù)據(jù)來(lái)源:光合作用文獻(xiàn) Endnote 數(shù)據(jù)庫(kù),更新至 2016 年 9 月,文獻(xiàn)數(shù)量超過(guò) 6000 篇

原始數(shù)據(jù)來(lái)源:Google Scholar

 

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Cooney, E. C. (2016). "The Effect of High-Intensity Visible Light on the Bloom Niches of the Phototrophic Dinoflagellates Alexandrium fundyense and Heterocapsa rotundata."

Gardner, S. G., et al. (2016). Dimethylsulfoniopropionate, superoxide dismutase and glutathione as stress response indicators in three corals under short-term hyposalinity stress. Proc. R. Soc. B, The Royal Society.

Hu, H., et al. (2016). "Effects of artificial sweeteners on metal bioconcentration and toxicity on a green algae Scenedesmus obliquus." Chemosphere 150: 285-293.

Jiang, Y., et al. (2016). "Towards elucidation of the toxic mechanism of copper on the model green alga Chlamydomonas reinhardtii." Ecotoxicology: 1-9.

Jin, J., et al. (2016). "Extracellular hydrocarbon and intracellular lipid accumulation are related to nutrient-sufficient conditions in pH-controlled chemostat cultures of the microalga Botryococcus braunii SAG 30.81." Algal Research 17: 244-252.

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Ritchie, R. J. and N. Mekjinda (2016). "Arsenic toxicity in the water weed Wolffia arrhiza measured using Pulse Amplitude Modulation Fluorometry (PAM) measurements of photosynthesis." Ecotoxicology and Environmental Safety 132: 178-185.

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