mcf7細胞

細胞名稱： 人乳腺癌細胞（STR鑒定正確）

細胞簡稱： MCF-7細胞

細胞別名： MCF 7; MCF.7; MCF-7；MCF7; Michigan Cancer Foundation-7; ssMCF-7;  ssMCF7; MCF7/WT; IBMF-7; MCF7-CTRL；

mcf7細胞貨號：TCH-C247

規格：1×10?cells/T25 培養瓶

參考價格：1500元  (干冰運輸需另加200元干冰費) 

種屬來源：人

組織來源：乳腺

特征：乳腺癌

細胞形態：上皮細胞樣

生長特性：貼壁生長

培養體系： MEM+10%FBS+1%Glutamax+1%NEAA+1% Sodium Pyruvate+10 µg/mL Human recombinant insulin+1%P/S

mcf7細胞 培養基 貨號：TCH-G247

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

培養條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎培養基+30%FBS+10%DMSO，液氮儲存

質量檢測：細菌、真菌、支原體檢測均為陰性

mcf7細胞 的生長受到腫瘤壞死因子α（TNF alpha）的抑制。可以通過用抗雌激素治療來調節IGFBP的分泌。

參考文獻：

Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111

Takahashi K, Suzuki K. Association of insulin-like growth-factor-I-induced DNA synthesis with phosphorylation and nuclear exclusion of p53 in human breast cancer MCF-7 cells. Int. J. Cancer 55: 453-458, 1993. PubMed: 8375929

Brandes LJ, Hermonat MW. Receptor status and subsequent sensitivity of subclones of MCF-7 human breast cancer cells surviving exposure to diethylstilbestrol. Cancer Res. 43: 2831-2835, 1983. PubMed: 6850594

Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

Pratt SE, Pollak MN. Estrogen and antiestrogen modulation of MCF7 human breast cancer cell proliferation is associated with specific alterations in accumulation of insulin-like growth factor-binding proteins in conditioned media. Cancer Res. 53: 5193-5198, 1993. PubMed: 7693333

Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088