Jurkat細胞
細胞名稱: 人T淋巴細胞白血*細胞(STR鑒定正確)
細胞簡稱:Jurkat細胞
細胞別稱:JurkatE6-1; Jurkat E6-1; Jurkat, Clone E6-1; Jurkat Clone E6-1; Jurkat (clone E6-1); JURKAT E-6.1; JURKAT E-61; Jurkat-E6; Jurkat E6; J.E6-1; E6-1
貨號:TCH-C225
規格: 1×10?cells/T25 培養瓶
價格:1500元 (干冰運輸需另加200元干冰費)
種屬來源:人
組織來源:外周血
特征:急性T淋巴細胞白血*
細胞形態:淋巴母細胞樣
生長特性:懸浮生長
培養體系:RPMI-1640+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S
Jurkat細胞 配套培養基貨號:TCH-G225
傳代比例:1:3-1:6,每2-3天換液一次
傳代周期:24-48 h
培養條件:氣相:95%空氣+5%CO2,溫度:37℃
凍存條件:60%基礎培養基+30%FBS+10%DMSO,液氮儲存
質量檢測:細菌、真菌、支原體檢測均為陰性
該細胞是Jurkat-FHCRC細胞株(Jurkat細胞株的衍生)的一個克隆。生長方式為懸浮生長,生長速度較快,使用1640培養液培養。顯微鏡下觀察,正常細胞狀態為圓潤透亮,似魚卵。
Jurkat細胞株來源于一個14歲男孩的外周血;經弗波酯和外源凝集素或抗T3單克隆抗體中的一種誘導后可產生大量IL-2(IL-2的產生需兩種類型的誘導劑);表達T細胞受體、CD3。
蘇州阿爾法生物實驗器材有限公司除了提供細胞系購買,細胞株購買,pc12細胞株,肺癌細胞株,Jurkat細胞,血管內皮細胞株,肝癌細胞株,腫瘤細胞株,胃癌細胞株,hela細胞株,胰島細胞株,s180細胞株,hela細胞株,動物細胞,干細胞外,還提供MEM培養基,DMEM,F12培養基,RPMI培養基等基礎培養基,干細胞培養基,培養基添加劑,間充質干細胞培養基 ,培養基,低血清培養基,胎牛血清,雞血清等細胞培養試劑。
參考文獻:
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