b16f10細胞-小鼠皮膚黑色素瘤細胞
b16f10細胞是B16-F0的亞系.
細胞名稱: 小鼠皮膚黑色素瘤細胞
簡稱:b16f10細胞
細胞別名: B16/F10; B16 F10; B16F10; B16 melanoma F10
貨號:TCM-C713
規格: 1X10^6
價格:140 0元 (干冰運輸需另加200元干冰費)
種屬來源:小鼠
組織來源:皮膚
特征:黑色素瘤
細胞形態:上皮細胞樣混有梭形細胞
生長特性:貼壁生長
培養體系:DMEM-H+10%FBS+1%P/S
b16f10細胞培養基貨號:TCM-G713
傳代比例:1:3-1:6,每2-3天換液一次
傳代周期:48-72 h
培養條件:氣相:95%空氣+5%CO2,溫度:37℃
b16f10細胞凍存條件:60%基礎培養基+30%FBS+10%DMSO,液氮儲存
質量檢測:細菌、真菌、支原體檢測均為陰性
參考文獻:
Fidler IJ. Biological behavior of malignant melanoma cells correlated to their survival in vivo. Cancer Res. 35: 218-224, 1975. PubMed: 1109790
Fidler IJ, et al. Tumoricidal properties of mouse macrophages activated with mediators from rat lymphocytes stimulated with concanavalin A. Cancer Res. 36: 3608-3615, 1976. PubMed: 953987
Fidler IJ, Bucana C. Mechanism of tumor cell resistance to lysis by syngeneic lymphocytes. Cancer Res. 37: 3945-3956, 1977. PubMed: 908034
Fidler IJ, Kripke ML. Metastasis results from preexisting variant cells within a malignant tumor. Science 197: 893-895, 1977. PubMed: 887927
Briles EB, Kornfeld S. Isolation and metastatic properties of detachment variants of B16 melanoma cells. J. Natl. Cancer Inst. 60: 1217-1222, 1978. PubMed: 418183
Fidler IJ. Selection of successive tumour lines for metastasis. Nat. New Biol. 242: 148-149, 1973. PubMed: 4512654
Li M, et al. Loss of intracisternal A-type retroviral particles in BL6 melanoma cells transfected with MHC class I genes. J.Gen. Virol. 77: 2757-2765, 1996. PubMed: 8922469
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