AsPc-1細(xì)胞-人轉(zhuǎn)移胰腺腺癌細(xì)胞

細(xì)胞名稱(chēng)： 人轉(zhuǎn)移胰腺腺癌細(xì)胞（STR鑒定正確）

細(xì)胞簡(jiǎn)稱(chēng)：AsPC-1細(xì)胞

細(xì)胞別名： AsPc-1; Aspc-1; ASPC-1; As-PC1; ASPC1; AsPC1; Aspc1; AsPc1

貨號(hào)：TCH-C128

規(guī)格： 1X10^6

價(jià)格：1200元 (干冰運(yùn)輸需另加200元干冰費(fèi))  

種屬來(lái)源：人

組織來(lái)源：胰腺

特征：轉(zhuǎn)移灶；腹水腺癌

細(xì)胞形態(tài)：上皮細(xì)胞樣

生長(zhǎng)特性：貼壁生長(zhǎng)

培養(yǎng)體系：RPMI-1640+10%FBS+1%P/S

AsPC-1細(xì)胞培養(yǎng)基貨號(hào)：TCH-G128

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

AsPC-1細(xì)胞 凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存

質(zhì)量檢測(cè)：細(xì)菌、真菌、支原體檢測(cè)均為陰性

人轉(zhuǎn)移胰腺腺癌細(xì)胞（AsPC-1)，取自62歲高加索人女性，該細(xì)胞系是合適的轉(zhuǎn)染宿主。

參考文獻(xiàn)：

Chen WH, et al. Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites. In Vitro 18: 24-34, 1982. PubMed: 7182348

Tan MH, et al. Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft. J. Natl. Cancer Inst. 67: 563-569, 1981. PubMed: 6944528

Loor R, et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982. PubMed: 6754226

Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

Tan MH, Chu TM. Characterization of the tumorigenic and metastatic properties of a human pancreatic tumor cell line (AsPC-1) implanted orthotopically into nude mice. Tumor Biol. 6: 89-98, 1985. PubMed: 4023565