?????????????????????? 3D Spheroids culture BioReactors

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16???????j??????????wu??????1??60rpm??????
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• ??????:?????,10mL??????,??????????μ????????????????(synthecon????RCCS????????軺?,???????????????,????
• ????????,??????????????????????????????????????в??)
• ?????????????·??
• ?????????????????????????????????????????????????????????RCCS?????????
• ??????:??????????????????г???300???????,?????80000-100000????????
• ???????:?????????????????????:????????????????invivo????
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• ????????:???????????????,??????????????壬????????????1-2mm(??????????й??,?????????????????
• ?????:?????????????invivo???
• ?????????????ж???????????????????????
• ???????????????????????????????????????(??RCCS?????????????)

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1)??????????????????RCCS??????????????

????????????????????????????????磬?????????3D?????????о???????????Ч??????????

2)??????(10 mL)??

RCCS????????????????軺????????????12 mL????????????????У????????????????????????????????????8 mL????????????????????????????????????????????в????????????????????????????10 mL?????????????????

3)?????????????ж????????????????????????
4)???????????????????????·??
???????????????????????????????????????????????????????????????????е?????????????300????RCCS?????????

BAM?????

1)BAM??16????????

????????????37?C??????????????RCCS??4???????????????????????????????????????????????????????????????????????????????RCCS??????????????????16????????????????????????????

2)BAM????????wu?????
???????????????????????????檔RCCS????????????????λ??????С?

3)BAM?????????
 BAM?????????????????????????????????á?

4)???????????????????????????????

RCCS????????趨??????????????????? 
5)???W-LAN????????????п???
RCCS???????/??е???? BAM????18?????????????????????

6)BAM?????????
??????????????仯??????????????洢???У???????????????????????????????????Ч??????????????????е??????)??RCCS??д????

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1. Metabolic Reprogramming and the Recovery of Physiological Functionality in 3D Cultures in Micro-Bioreactors
   Krzysztof Wrzesinski and Stephen J. Fey
   Bioengineering, 5 (2) 1-25: 2018 
   DOI: 10.3390/bioengineering5010022
2. 
    
   
3. Recent advances in three-dimensional cell culturing to assess liver function and dysfunction: from a drug biotransformation and toxicity perspective.
   Carlemi Calitz, Josias H. Hamman, Stephen J. Fey, Krzysztof Wrzesinski & Chrisna Gouws
   Toxicology Mechanisms and Methods, 2018
   
4. Acetaminophen-induced S-nitrosylation and S-sulfenylation signalling in 3D cultured hepatocarcinoma cell spheroids.
   K. Wojdyla, K. Wrzesinski, J. Williamson, P. Roepstorff, S.J. Fey, A. Rogowska-Wrzesinska 
   Toxicology Research 5(2) 905-920, 2016 
   
5. From 2D to 3D - a new dimension for modelling the effect of natural products on human tissue. 
   K. Wrzesinski and S.J. Fey
   Current Pharmaceutical Design 21(38): 5605-5616, 2015. 
   PMID:  
   DOI: 10.2174/1381612821666151002114227
6. Top-down and Middle-down Protein Analysis Reveals that Intact and Clipped Human Histones Differ in Post-translational Modification Patterns. 
   A. Tvardovskiy, K. Wrzesinski, S. Sidoli, S.J. Fey, A. Rogowska-Wrzesinska, O.N. Jensen
   Molecular and Cellular Proteomics 14(12):3142-53; 2015. 
   PMID:  
   DOI: 10.1074/mcp.M115.048975
   The cultural divide: exponential growth in classical 2D and metabolic equilibrium in 3D environments.
   K. Wrzesinski, A. Rogowska-Wrzesinska, R. Kanlaya, K. Borkowski, V. Schw?mmle, J. Daia, K.E. Joensen, K. Wojdyla, V. Botelho Carvalho & S.J. Fey 
   PLOS One 9(9) 1-15; 2014
   PMID:  
   DOI: 10.1371/journal.pone.0106973The cultural divide: exponential growth in classical 2D and metabolic equilibrium in 3D environments.
   K. Wrzesinski, A. Rogowska-Wrzesinska, K. Borkowski, V. Botelho Carvalho & S.J. Fey 
   Poster at 9th Danish Conference on Biotechnology and Molecular Biology, Vejle; 2014 
   DOI: 10.13140/2.1.2643.2965
7. Heteromer score ?C using internal standards to assess the quality of proteomic data.
   A. Rogowska-Wrzesinska, K. Wrzesinski and S.J. Fey
   Proteomics. 14(9):1042-7 2014
   PMID:  
   DOI: 10.1002/pmic.201300457
   
8. Microgravity spheroids as a reliable, long term tool for predictive toxicology. 
   S.J. Fey and K. Wrzesinski
   Toxicology Letters, 221S S153 2013. 
   DOI: 10.1016/j.toxlet.2013.05.318
9. Determination of acute lethal and chronic lethal dose thresholds of Valproic acid using 3D spheroids constructed from the immortal human hepatocyte cell line HepG2/C3A. 
   S.J. Fey and K. Wrzesinski
   In ''Valproic Acid: Pharmacology, Mechanisms of Action and Clinical Implications'' Nova Science Publishers, New York. Ch. V, 141-165; 2013
10. Human liver spheroids exhibit stable physiological functionality for at least 24 days after recovering from trypsinisation.
    K. Wrzesinski, C.M. Magnone, L. Visby Hansen, M. Ehrhorn Kruse, T. Begauer, M. Bobadilla, M. Gubler, J. Mizrahi, C. M?ller Andreasen, K. Zhang, K. Eyed Joensen, S.M. Andersen and S.J. Fey
    Toxicology Research; 2(3) 163-172; 2013 
    DOI: 10.1039/C3TX20086H 
    NB: Manuscript featured on the front cover of the journal.
    
11. After trypsinisation, 3D spheroids of C3A hepatocytes need 18 days to re-establish similar levels of key physiological functions to those seen in the liver.
    K. Wrzesinski and S.J. Fey
    Toxicology Research; 2(2) 123-135; 2013 
    DOI: 10.1039/C2TX20060K
    NB: Manuscript featured on the front cover of the journal.
    
12. Determination of drug toxicity using 3D spheroids constructed from immortalized human hepatocytes. 
    S.J. Fey and K. Wrzesinski
    Toxicological Sciences 127(2) 403-411; 2012. 
    PMID:  
    DOI: 10.1093/toxsci/kfs122

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