新wu應力旋轉微重力培養

生物反應器點

1)該生物反應器可以打開，而RCCS生物反應器不可以。

此特點可使實驗人員隨時收集或處理樣本。例如，可用藥物處理3D組織，然后研究其對擬組織的效應和恢復情況。

2)體積固定(10 mL)。

RCCS生物反應器配備了可拉伸硅膠膜，灌注體積高達12 mL。在細胞培養過程中，水分會從膜內蒸發，在出現明顯氣泡前體積可降至8 mL。此時培養基離子強度會顯著增加，擬組織內的細胞會受到脅迫。該生物反應器采用了剛性膜，體積為10 mL，不會發生這種情況。

3)生物反應器側面帶有端口，便于更換培養基和除氣泡。

4)該生物反應器內置水合腔和加室通路。
此特點可使其置于干燥的孵箱內使用，減少細菌和支原體污染。在長期培養時這點尤其重要（有的實驗培養時間超過300天）。RCCS沒有水合腔。

BAM單元點

1)BAM有16個立驅動

驅動單元冷卻在37?C以免影響孵箱溫度。RCCS系統4個驅動。如果要在同一個孵箱內使用兩個單元，需要購買一個能夠冷卻和加熱的孵箱。目前尚沒有能夠同時冷卻四個RCCS單元的孵箱。如果要同時進行16個培養，需要購買另外的可冷卻孵箱。

2)BAM由平板電腦wu線控制
戴上手套可很方便地調節速度和清理表面。RCCS則是采用了一個帶電位計的控制盒。

3)BAM轉動非常平滑
BAM旋轉時的抖動顯著減少，因此轉速低時也可以使用。

4)顯示器顯示的是驅動單元馬達軸的真實轉速

RCCS顯示的是設定的轉速，真實轉速可能不同。
5)通過W-LAN通訊和安卓界面進行控制
RCCS系統是電子/機械式的。 BAM帶有18個微電腦（不含平板電腦）。

6)BAM帶有日志系統
時間和驅動桿設置變化都記錄在一個只讀存儲器中（用戶不能改變記錄）。這種功能可以用于產生績效文件（如用于制藥工業中的藥物開發)。RCCS沒有此特點。

文獻：

Publications

Metabolic Reprogramming and the Recovery of Physiological Functionality in 3D Cultures in Micro-Bioreactors
Krzysztof Wrzesinski and Stephen J. Fey
Bioengineering, 5 (2) 1-25: 2018
DOI: 10.3390/bioengineering5010022
Recent advances in three-dimensional cell culturing to assess liver function and dysfunction: from a drug biotransformation and toxicity perspective.
Carlemi Calitz, Josias H. Hamman, Stephen J. Fey, Krzysztof Wrzesinski & Chrisna Gouws
Toxicology Mechanisms and Methods, 2018
Acetaminophen-induced S-nitrosylation and S-sulfenylation signalling in 3D cultured hepatocarcinoma cell spheroids.
K. Wojdyla, K. Wrzesinski, J. Williamson, P. Roepstorff, S.J. Fey, A. Rogowska-Wrzesinska
Toxicology Research 5(2) 905-920, 2016
From 2D to 3D - a new dimension for modelling the effect of natural products on human tissue.
K. Wrzesinski and S.J. Fey
Current Pharmaceutical Design 21(38): 5605-5616, 2015.
PMID:
DOI: 10.2174/1381612821666151002114227
Top-down and Middle-down Protein Analysis Reveals that Intact and Clipped Human Histones Differ in Post-translational Modification Patterns.
A. Tvardovskiy, K. Wrzesinski, S. Sidoli, S.J. Fey, A. Rogowska-Wrzesinska, O.N. Jensen
Molecular and Cellular Proteomics 14(12):3142-53; 2015.
PMID:
DOI: 10.1074/mcp.M115.048975
The cultural divide: exponential growth in classical 2D and metabolic equilibrium in 3D environments.
K. Wrzesinski, A. Rogowska-Wrzesinska, R. Kanlaya, K. Borkowski, V. Schw?mmle, J. Daia, K.E. Joensen, K. Wojdyla, V. Botelho Carvalho & S.J. Fey
PLOS One 9(9) 1-15; 2014
PMID:
DOI: 10.1371/journal.pone.0106973 The cultural divide: exponential growth in classical 2D and metabolic equilibrium in 3D environments.
K. Wrzesinski, A. Rogowska-Wrzesinska, K. Borkowski, V. Botelho Carvalho & S.J. Fey
Poster at 9th Danish Conference on Biotechnology and Molecular Biology, Vejle; 2014
DOI: 10.13140/2.1.2643.2965
Heteromer score – using internal standards to assess the quality of proteomic data.
A. Rogowska-Wrzesinska, K. Wrzesinski and S.J. Fey
Proteomics. 14(9):1042-7 2014
PMID:
DOI: 10.1002/pmic.201300457
Microgravity spheroids as a reliable, long term tool for predictive toxicology.
S.J. Fey and K. Wrzesinski
Toxicology Letters, 221S S153 2013.
DOI: 10.1016/j.toxlet.2013.05.318
Determination of acute lethal and chronic lethal dose thresholds of Valproic acid using 3D spheroids constructed from the immortal human hepatocyte cell line HepG2/C3A.
S.J. Fey and K. Wrzesinski
In ''Valproic Acid: Pharmacology, Mechanisms of Action and Clinical Implications'' Nova Science Publishers, New York. Ch. V, 141-165; 2013
Human liver spheroids exhibit stable physiological functionality for at least 24 days after recovering from trypsinisation.
K. Wrzesinski, C.M. Magnone, L. Visby Hansen, M. Ehrhorn Kruse, T. Begauer, M. Bobadilla, M. Gubler, J. Mizrahi, C. M?ller Andreasen, K. Zhang, K. Eyed Joensen, S.M. Andersen and S.J. Fey
Toxicology Research; 2(3) 163-172; 2013
DOI: 10.1039/C3TX20086H
NB: Manuscript featured on the front cover of the journal.
After trypsinisation, 3D spheroids of C3A hepatocytes need 18 days to re-establish similar levels of key physiological functions to those seen in the liver.
K. Wrzesinski and S.J. Fey
Toxicology Research; 2(2) 123-135; 2013
DOI: 10.1039/C2TX20060K
NB: Manuscript featured on the front cover of the journal.
Determination of drug toxicity using 3D spheroids constructed from immortalized human hepatocytes.
S.J. Fey and K. Wrzesinski
Toxicological Sciences 127(2) 403-411; 2012.
PMID:
DOI: 10.1093/toxsci/kfs122