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南京卡米洛生物工程有限公司
主營產品： 小鼠IgA類單克隆抗體腹水純化試劑盒,人17α-羥孕酮,豬雌二醇,白介素1受體拮抗劑 |
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祝賀華中農業大學老師使用南京卡米洛ELISA試劑盒發表分文章獲高分錄取！
2023-5-19 閱讀(796)
| 文獻標題 | GPA peptide enhances Nur77 expression in intestinal epithelial cells to exert a protective effect against DSS-induced colitis | ||||
| 文獻作者 | Zhao Deng1、Liufeng Zheng、Xiaowei Xie1、Hongkui Wei、Jian Peng | ||||
| 期刊名稱 | FASEB Journal | 影響因子 | 5.191 | ||
| 使用產品 | 小鼠髓過氧化物酶(Myeloperoxidase ,MPO)ELISA試劑盒 | 貨號 | 2M-KMLJM220987m | ||
| 小鼠活性氧(reactive oxygen species, ROS)ELISA試劑盒 | 2M-KMLJM219615m | ||||
| 人活性氧(reactive oxygen species, ROS)ELISA試劑盒 | 2H-KMLJh310725 | ||||
Abstract
Ulcerative colitis (UC) is a widespread inflammatory bowel disease that causes longlasting inflammation and ulcers in the colon and rectum. In the inflamed tissue of patients with UC, the tight junctions are disrupted and large amounts of pro-inflammatory cytokines are produced, resulting in immune dysregulation. The expression of Nur77 is significantly reduced in the colon of inflammatory bowel disease, while Nur77 deficiency increases the susceptibility to DSS-induced colitis. Here, we report that Gly-Pro-Ala (GPA) peptide isolated from fish skin gelatin hydrolysate can significantly alleviate intestinal inflammation and damage caused by DSS-induced mice colitis. Besides maintaining the intestinal epithelial barrier, GPA alleviates intestinal inflammation and oxidative stress by inhibiting NF-κB activation. Interestingly, GPA binds to the ligand-binding domain of Nur77 and stimulates its autotranscriptional activity to enhance its expression in intestinal epithelial cells. Furthermore, GPA activates the promoter of IκBα to increase its expression, resulting in the abolishment of the NF-κB pathway. In contrast, the inhibitory effects of GPA on colitis are abolished in Nur77-/- mice. Our results suggest that as a Nur77 modulator, GPA may be applied to the prevention of intestinal inflammation
