REAGENTS PROVIDED AND RECONSTTTUTION
REAGENTS(store at2-8℃) | 1×96WELLS | 0.5×96WELLS | RECONSTTTUTION |
96/48-wells miorou | 1 | 0.5 | Ready-to-use |
Plastiv cover | 2 | 0.5 | Ready-to-use |
Standard:1000pg/ml | 1Vials (0.6ml) | 0.5Vials (0.3ml) | See reagents preparation on page 3 |
Blank control | 1Vials (1.0ml) | 1Vials (0.5ml) | Ready-to-use |
Standard Diluent | 1Vials (5ml) | 1Vials (2.5ml) | Ready-to-use |
Biotinylated anti-ET-1 | 1Vials (6ml) | 1Vials (3.0ml) | Ready-to-use |
Streptavidin-HRP | 1Vials (10ml) | 1Vials (5.0ml) | Ready-to-use |
Washing Buffer | 1Vials (20ml) | 1Vials (10ml) | 50× concentrate |
Substrate A | 1Vials (6.0ml) | 1Vials (3.0ml) | Ready-to-use |
Substrate B | 1Vials (6.0ml) | 1Vials (3.0ml) | Ready-to-use |
Stopping Solution | 1Vials (6.0ml) | 1Vials (3.0ml) | Ready-to-use |
Sample Diluent | 1Vials (12ml) | 1Vials (6.0ml) | Ready-to-use |
MATERIAL REQUIRED BUT NOT PROVIDED
● Distilled water
● Pipettes:10ul、50ul、100ul、200ul、1000ul。
● Vortex mixer and magnetic stirrer.
SAFETY
● For research use only
● Avoid any skin contact with H2SO4 and TMB.In case of contact,wash thoroughly water.
● Do not eat,drink,smoke or apply cosmetics where kit reagents are used.
● Do not pipette by mouth
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