上海研盟生物科技有限公司Anti-PGRPS抗體*,主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發送,您也可以直接我司在線客服索取。客服
英文名稱:Anti-PGRPS antibody
中文名稱:肽聚糖識別蛋白1抗體
肽聚糖識別蛋白1抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
肽聚糖識別蛋白1抗體,Anti-PGRPS抗體抗原的分類:
一. 免疫學分類:
(1)*抗原、半抗原;
(2)胸腺依賴抗原與非依賴抗原。
二. 臨床分類:
(1)外源性抗原:微生物、花粉等;
(2)內源性抗原:隱蔽的自身抗原、腫瘤相關抗原等;
(3)同種異型抗原:人類白細胞抗原、血型抗原;
(4)異嗜性抗原:人與其他動物、植物等之間存在的共同抗原。
★研盟生物★肽聚糖識別蛋白1抗體,Anti-PGRPS抗體
Western Blotting 實驗步驟概述
1、 組織取材:組織塊稱重
2、 利用液氮、研缽粉碎組織塊
3、 加入RIPA緩沖液(每克組織3ml RIPA),PMSF(每克組織30μl,10 mg/ml PMSF),
利用Polytron進一步勻漿(15,000轉/分*1分鐘)維持4℃
4、 加入PMSF(每克組織30μl,10 mg/ml PMSF),冰上孵育30分鐘
5、 移入離心管4℃,約20,000g(約15,000轉)15分鐘
6、 上清液為細胞裂解液可分裝-20℃保存
7、 進行Bradford比色法測定蛋白質濃度
8、 取相同質量的細胞裂解液(體積*蛋白質濃度),并加等體積的2×電泳加樣緩沖液
9、 沸水浴中3分鐘
10、上樣
11、電泳(濃縮膠20mA,分離膠35mA)
12、電轉膜儀轉膜(100mA 40分鐘)
13、膜用麗春紅染色,膠用考馬斯亮藍染色
14、Westernblot 試劑盒顯色
15、分析比較記錄
★研盟生物 ★合作: ★客服
相關標記抗體:HRP標記抗體,Biotin標記抗體,Gold標記抗體,RBITC標記抗體,AP標記抗體,FITC標記抗體,Cy3標記抗體,Cy5標記抗體,Cy5.5標記抗體,Cy7標記抗體,PE標記抗體,PE-Cy3標記抗體,PE-Cy5標記抗體,PE-Cy5.5標記抗體,PE-Cy7標記抗體,APC標記抗體,Alexa Fluor 350標記抗體,Alexa Fluor 488標記抗體,Alexa Fluor 555標記抗體,Alexa Fluor 647標記抗體
簡單介紹:
產品別名:Peptidoglycan recognition protein 1; Peptidoglycan recognition protein; PGLYRP; PGLYRP1; PGRP; PGRP S; PGRP-PEN; PGRP-S; TAG7; TNFSF 3L; TNFSF3L; PGRP1_HUMAN.
抗體來源:Rabbit or Mouse
保質期:1年
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:The primary immune recognition is based on structures common among invading pathogens. Bacterial surface molecules, such as lipopolysaccharide (LPS) and peptidoglycan (PGN), are known to elicit immune reactions ranging from cytokine release to fever. Recently, a family of proteins called peptidoglycan recognition protein (PGRP) has been identified in mouse and human that binds to peptidoglycans expressed on Gram-positive bacteria. Peptidoglycan (PGN) is an essential cell wall component of virtually all bacteria and, thus, it is an excellent target for recognition by the eukaryotic innate immune system. The PGRPs (PGRP-L, PGRPS, PGRPIA, and PGRP-I-beta) define a new family of human pattern recognition molecules. PGRP-L is primarily expressed in the liver. Although liver is not considered a primary immune organ, liver participates in host defenses by producing acute phase proteins (by hepatocytes) in response to infections and by clearing microorganisms from blood. PGRPS is present in neutrophils and inhibits growth of Gram-positive bacteria and, therefore, may function as a neutrophil antibacterial protein. However, PGRPS may have another, as yet unidentified function because in humans it is expressed in the bone marrow 50–100 times higher than in neutrophils.
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