上海研盟生物科技有限公司Anti-Porimin抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取。客服
英文名稱:Anti-Porimin antibody
中文名稱:前細(xì)胞脹亡受體誘導(dǎo)膜損傷蛋白抗體
前細(xì)胞脹亡受體誘導(dǎo)膜損傷蛋白抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
前細(xì)胞脹亡受體誘導(dǎo)膜損傷蛋白抗體,Anti-Porimin抗體抗原的分類:
一. 免疫學(xué)分類:
(1)*抗原、半抗原;
(2)胸腺依賴抗原與非依賴抗原。
二. 臨床分類:
(1)外源性抗原:微生物、花粉等;
(2)內(nèi)源性抗原:隱蔽的自身抗原、腫瘤相關(guān)抗原等;
(3)同種異型抗原:人類白細(xì)胞抗原、血型抗原;
(4)異嗜性抗原:人與其他動(dòng)物、植物等之間存在的共同抗原。
★研盟生物★前細(xì)胞脹亡受體誘導(dǎo)膜損傷蛋白抗體,Anti-Porimin抗體
Western Blotting 實(shí)驗(yàn)步驟概述
1、 組織取材:組織塊稱重
2、 利用液氮、研缽粉碎組織塊
3、 加入RIPA緩沖液(每克組織3ml RIPA),PMSF(每克組織30μl,10 mg/ml PMSF),
利用Polytron進(jìn)一步勻漿(15,000轉(zhuǎn)/分*1分鐘)維持4℃
4、 加入PMSF(每克組織30μl,10 mg/ml PMSF),冰上孵育30分鐘
5、 移入離心管4℃,約20,000g(約15,000轉(zhuǎn))15分鐘
6、 上清液為細(xì)胞裂解液可分裝-20℃保存
7、 進(jìn)行Bradford比色法測(cè)定蛋白質(zhì)濃度
8、 取相同質(zhì)量的細(xì)胞裂解液(體積*蛋白質(zhì)濃度),并加等體積的2×電泳加樣緩沖液
9、 沸水浴中3分鐘
10、上樣
11、電泳(濃縮膠20mA,分離膠35mA)
12、電轉(zhuǎn)膜儀轉(zhuǎn)膜(100mA 40分鐘)
13、膜用麗春紅染色,膠用考馬斯亮藍(lán)染色
14、Westernblot 試劑盒顯色
15、分析比較記錄
★研盟生物 ★合作: ★客服
相關(guān)標(biāo)記抗體:HRP標(biāo)記抗體,Biotin標(biāo)記抗體,Gold標(biāo)記抗體,RBITC標(biāo)記抗體,AP標(biāo)記抗體,FITC標(biāo)記抗體,Cy3標(biāo)記抗體,Cy5標(biāo)記抗體,Cy5.5標(biāo)記抗體,Cy7標(biāo)記抗體,PE標(biāo)記抗體,PE-Cy3標(biāo)記抗體,PE-Cy5標(biāo)記抗體,PE-Cy5.5標(biāo)記抗體,PE-Cy7標(biāo)記抗體,APC標(biāo)記抗體,Alexa Fluor 350標(biāo)記抗體,Alexa Fluor 488標(biāo)記抗體,Alexa Fluor 555標(biāo)記抗體,Alexa Fluor 647標(biāo)記抗體
簡(jiǎn)單介紹:
產(chǎn)品別名:KCT3; Keratinocytes associated transmembrane protein 3; Pro oncosis receptor inducing membrane injury; Serine/threonine rich receptor; TMEM123; Transmembrane protein 123; PORIM_HUMAN.
抗體來源:Rabbit or Mouse
保質(zhì)期:1年
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:This gene encodes a highly glycosylated transmembrane protein with a high content of threonine and serine residues in its extracellular domain, similar to a broadly defined category of proteins termed mucins. Exposure of some cell types to anti PORIMIN (pro oncosis receptor inducing membrane injury) antibody, crosslinks this protein on the cell surface and induces a type of cell death termed oncosis. Oncosis is distinct from apoptosis and is characterized by a loss of cell membrane integrity without DNA fragmentation. This gene product is proposed to function as a cell surface receptor that mediates cell death.
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