★研盟生物 ★合作: ★客服
相關標記抗體:HRP標記抗體,Biotin標記抗體,Gold標記抗體,RBITC標記抗體,AP標記抗體,FITC標記抗體,Cy3標記抗體,Cy5標記抗體,Cy5.5標記抗體,Cy7標記抗體,PE標記抗體,PE-Cy3標記抗體,PE-Cy5標記抗體,PE-Cy5.5標記抗體,PE-Cy7標記抗體,APC標記抗體,Alexa Fluor 350標記抗體,Alexa Fluor 488標記抗體,Alexa Fluor 555標記抗體,Alexa Fluor 647標記抗體
上海研盟生物科技有限公司Anti-NVL抗體*,主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發送,您也可以直接我司在線客服索取??头?/span>
英文名稱:Anti-NVL antibody
中文名稱:核VCP樣蛋白抗體
核VCP樣蛋白抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
★研盟生物★核VCP樣蛋白抗體,Anti-NVL抗體
免疫組化操作步驟
(一)、儀器設備
1、 18cm不銹鋼高壓鍋或電爐或用微波爐
2、 水浴鍋
(二)、試 劑
1、 PBS緩沖液(pH7.2―7.4)
2、 0.01mol/L檸檬酸鈉緩沖液(CB,pH6.0,1000ml)
3、 3%甲醇―H2O2溶液:用30% H2O2 和80%甲醇溶液配制
4、 風裱劑:
a. 甘油和0.5mmol/L碳酸鹽緩沖液(pH9.0–9.5)等量混合
b. 油和TBS(PBS)配制
5、 TBS/PBS pH9.0–9.5,適用于熒光纖維鏡標本;pH7.0-7.4適合光學纖維標本
(三)、操作流程
1、 脫蠟和水化:脫蠟前應將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘
a. 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b. 無水乙醇中浸泡五分鐘
c. 95%乙醇中浸泡五分鐘
d. 75%乙醇中浸泡五分鐘
2、 抗原修復:用于福爾馬林固定的石蠟包埋組織芯片
核VCP樣蛋白抗體,Anti-NVL抗體溶解方法:
方法1:我們的抗體(凍干粉)已經包含了0.01M PBS、1% BSA和0.1% 防腐劑(疊氮鈉或*),您只需要加入滅菌的雙蒸水就行了。抗體溶解后,置于-20℃保存,分裝后使用,避免反復凍融,可保證至少1年有效;
方法2:也可以只加入一半體積的雙蒸水,再用一半體積的甘油補足,置于-20℃保存,這樣抗體不會凍,有利于抗體的穩定。
后續試驗時,再使用對應的緩沖液稀釋成工作液,即用即配。
我們的抗體(凍干粉),在常溫放置下,至少一個月有效;若在-20℃下保存(*),至少三年有效。
Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:
1) Reconstituting by directly adding 100uL of sterile, distilled water.
2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.
簡單介紹:
產品別名:Nuclear valosin containing protein like; Nuclear valosin-containing protein-like; Nuclear VCP like; Nuclear VCP-like protein; Nvl; NVL_HUMAN; NVLp; OTTHUMP00000035641; OTTHUMP00000216593; OTTHUMP00000216594; OTTHUMP00000216599; OTTHUMP00000216600.
抗體來源:Rabbit or Mouse
保質期:1年
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:This gene encodes a member of the AAA (ATPases associated with diverse cellular activities) superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. Two encoded proteins, described as major and minor isoforms, have been localized to distinct regions of the nucleus. The largest encoded protein (major isoform) has been localized to the nucleolus and shown to participate in ribosome biosynthesis (PMID: 15469983, 16782053), while the minor isoform has been localized to the nucleoplasmin.
相關優質產品:
SMN1(survival of motor neuron 1) 運動神經元生存蛋白1抗體
SLT/SLT-IIe(O139)(Shiga-Like Toxin Type II Variant) 豬水腫素(O139)抗體
SLC39A6(solute carrier family 39, member 6) SLC39A6抗體
Slug 鋅指轉錄因子Slug抗體
Smad 1(MotheBYK against decapentaplegic homolog 1) Smad 1抗體
Smad2/3(MotheBYK against decapentaplegic homolog 2) Smad 2/3抗體
Smad4(MotheBYK against decapentaplegic homolog 4) Smad4抗體
Smad7(MotheBYK against decapentaplegic homolog 7) Smad 7抗體
phosphor-SMAD(p-Ser425)、phosphor-MotheBYK against decapentaplegic 磷酸化SMAD抗體
SM-MHC(Cardiac myosin heavy chain) 心肌肌凝蛋白抗體
SMMHC/MYH11 平滑肌肌球蛋白重鏈抗體
SM22α(smooth muscle 22 alpha) 細胞骨架相關蛋白SM22α抗體
Regucalcin/SMP30/Rgn(Senescence marker protein 30) 衰老標記蛋白30抗體
Snake poison Protein 抗蛇毒蛋白抗體(蝮蛇)
Snake poison Protein 抗蛇毒蛋白抗體(中華眼鏡蛇)
SNAP-25 (synaptosomal-associated protein 25) 突觸相關蛋白25抗體
Snail 抗Snail蛋白抗體
Socs 1 (suppressor of cytokine signaling 1) 細胞因子信號傳導抑制蛋白1抗體
Socs 2(suppressor of cytokine signaling 2) 細胞因子信號傳導抑制蛋白2抗體
Socs 3 (suppressor of cytokine signaling 3) 細胞因子信號傳導抑制蛋白3抗體