中文名稱:肌肉特定環指蛋白3抗體
英文名稱:Anti-MURF3 antibody
肌肉特定環指蛋白3抗體,Anti-MURF3抗體背景介紹:MuRF1, is a nuclear protein that interacts with SMT3b and the large myofibrillar protein Titin. In muscle cells, MuRF2 (RFN29) regulates gene expression and protein turnover. It localizes to the cytoplasm, but under atrophic conditions it is detected in the nucleus. MuRF2 can form oligomers with various other proteins, including Titin and Myosin. MuRF3, also designated tripartite motif-containing 54 (TRIM54) or ring finger protein 30 (RNF30), interacts with tubulin and stabilizes microtubules duing myotube formation. It is a cytoplasmic protein the localizes to the Z-lines in skeletal muscles, while MuRF2 localizes to the sarcomeric M-band in cardiomyocytes. MuRF3 shares 77% and 65% sequence identity with MuRF1 and MuRF2, respectively. MuRF1-3 share a conserved N-terminal RING domain and zinc-binding B-box motif, and two coiled-coil dimerization motif boxes, in their central regions.
上海研盟生物有限公司*品質供應Anti-MURF3抗體,我公司銷售國內外科研產品,如生物試劑、生化試劑,一抗二抗,各種標記抗體,Elisa酶聯免疫試劑盒,放免試劑盒,培養基,各種動物血清等產品,歡迎廣大新老客戶前來咨詢訂購,訂購:或。
相關標記抗體:HRP標記抗體,Biotin標記抗體,Gold標記抗體,RBITC標記抗體,AP標記抗體,FITC標記抗體,Cy3標記抗體,Cy5標記抗體,Cy5.5標記抗體,Cy7標記抗體,PE標記抗體,PE-Cy3標記抗體,PE-Cy5標記抗體,PE-Cy5.5標記抗體,PE-Cy7標記抗體,APC標記抗體,Alexa Fluor 350標記抗體,Alexa Fluor 488標記抗體,Alexa Fluor 555標記抗體,Alexa Fluor 647標記抗體
保質期: 1年
肌肉特定環指蛋白3抗體,Anti-MURF3抗體簡單介紹:
產品別名:MURF 3; MURF; MURF-3; MuRF3; Muscle specific RING finger protein 3; Muscle specific RING finger protein; Muscle specific RING finger protein homolog; Muscle-specific RING finger protein 3; Muscle-specific RING finger protein; Ring finger protein 30; RNF30; TRI54_HUMAN; TRIM 54; TRIM54; RNF28; Tripartite motif containing 54; tibody Tripartite motif-containing protein 54.
抗體來源:Rabbit or Mouse
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
肌肉特定環指蛋白3抗體抗原抗體反應:
一、抗原抗體反應特點
1.高度特異性,特異性是抗原抗體反應的zui主要特征,特定抗原決定簇只與特定抗體結合;
2.可逆性:抗原與抗體結合形成抗原抗體復合物的過程是一種動態平衡, 其反應式為:Ag+AbAg·Ab;
3.適當濃度和比例;
4.敏感性:在測定血清中某一物質的含量時,化學比色法的敏感度為mg/ml水平,酶反應測定法的敏感度約為5~10μg/ml,免疫測定中凝膠擴散法的敏感度與酶反應法相仿。
二、影響抗原抗體反應的因素
1.電解質 :抗原抗體特異結合后,膠體粒便可發生脫水作用,此時易受電解質影響,如有適當濃度的電解質存在就會使它們失去部分負電荷而相互凝聚,于是出現明顯的凝集或沉淀現象。
2.酸堿度:合適的pH為6-8。
3.溫度:37℃通常是抗原體反應zui適溫度。
免疫組化染色失敗的幾種原因
1.所染的全部切片均為陰性結果,包括陽性對照在內,全部呈陰性反應,原因可能是:(1)染色未按嚴格操作步驟進行;(2)漏加一種抗體,或抗體失效;(3)緩沖液內含*,抑制了酶的活性;(4)底物中所加H2O2量少或失效;復染或脫水劑使用不當。
2.所有切片均呈弱陽性反應:(1)切片在染色過程中抗體過濃,或干燥了;(2)緩沖液配制中未加氯化鈉或pH不準確,洗滌不*;(3)使用已變色的呈色底物溶液,或呈色反應時間過長;(4)抗體溫育的時間過長;(5)H2O2濃度過高,呈色速度過快;(6)粘附劑太厚。
3.所有切片背景過深:(1)未用酶消化處理切片;(2)切片或涂片過厚;(3)漂洗不夠;(4)底物呈色反應過久;(5)蛋白質封閉不夠或所用血清溶血;(6)使用全血清抗體稀釋不夠。
4.陽性對照染色良好,檢測的陽性標本呈陰性反應,固定和處理不當是zui常見的原因。對于陽性結果的定量判斷,常規方法是根據呈色深淺和陽性細胞數量分類計數,以(-)、+、++、+++等分級和計數統計。
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