中文名稱(chēng):溶質(zhì)載體家族蛋白34成員A3抗體
英文名稱(chēng):Anti-SLC34A3 antibody
上海研盟生物科技有限公司Anti-SLC34A3抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說(shuō)明書(shū)隨貨發(fā)送,您也可以直接我司在線客服索取。客服:.
溶質(zhì)載體家族蛋白34成員A3抗體,Anti-SLC34A3抗體簡(jiǎn)單介紹:
產(chǎn)品別名:HHRH; Na(+) dependent phosphate cotransporter 2C; Na(+) Pi cotransporter 2C; Na(+)-dependent phosphate cotransporter 2C; Na(+)/Pi cotransporter 2C; NaPi 2c; NaPi-2c; NPT2C; NPT2C_HUMAN; NPTIIC; SLC34A3; Sodium dependent phosphate transport protein 2C; Sodium inorganic phosphate cotransporter IIC; Sodium phosphate cotransporter 2C; Sodium phosphate transport protein 2C; Sodium-dependent phosphate transport protein 2C; Sodium-phosphate transport protein 2C; Sodium/inorganic phosphate cotransporter IIC; Sodium/inorganic phosphate cotransporter, type IIC; Sodium/phosphate cotransporter 2C; solute carrier family 34 (sodium phosphate) member 3; solute carrier family 34 (sodium/phosphate contransporter), member 3; solute carrier family 34 (type II sodium/phosphate contransporter), member 3; Solute carrier family 34 member 3; type IIc Na+ Pi cotransporter.
產(chǎn)品規(guī)格:0.1ml、0.2ml、1ml
產(chǎn)品用途:科研實(shí)驗(yàn)
產(chǎn)品價(jià)格:請(qǐng)報(bào)價(jià)
說(shuō)明書(shū):請(qǐng)?zhí)砑涌头苯铀魅?/p>
抗體來(lái)源:該指標(biāo)有兩種產(chǎn)品,一種是兔來(lái)源抗體,一種是鼠來(lái)源抗體
克隆類(lèi)型:兔來(lái)源為多克隆抗體,鼠來(lái)源單克隆抗體
交叉反應(yīng):請(qǐng)索取說(shuō)明書(shū)查看
背景介紹:This gene encodes a member of SLC34A transporter family of proteins, and is expressed primarily in the kidney. It is involved in transporting phosphate into cells via sodium cotransport in the renal brush border membrane, and contributes to the maintenance of inorganic phosphate concentration in the kidney. Mutations in this gene are associated with hereditary hypophosphatemic rickets with hypercalciuria. Alternatively spliced transcript variants varying in the 5' UTR have been found for this gene.
溶質(zhì)載體家族蛋白34成員A3抗體保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲(chǔ) 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
免疫應(yīng)答過(guò)程:
1.抗原進(jìn)入機(jī)體;
2.抗原刺激 B淋巴細(xì)胞,使之分化增殖成為抗體分泌漿細(xì)胞和記憶細(xì)胞(初次免疫應(yīng)答);
3.抗體主要由漿細(xì)胞分泌 ;
4.記憶細(xì)胞再次受到特異性抗原刺激后會(huì)被激活而成為分泌抗體的漿細(xì)胞(再次免疫應(yīng)答);
5.記憶B細(xì)胞是長(zhǎng)命細(xì)胞,可以存活數(shù)月甚至機(jī)體終生 。
初次體液免疫是指宿主*次接觸外來(lái)TD抗原后血清中逐漸出現(xiàn)抗原特異性抗體的過(guò)程。初次體液免疫應(yīng)答的延遲相(從抗原免疫到抗體水平達(dá)到高峰的時(shí)間)為6-10天,起初幾天血清中的抗原特異性抗體主要為親和力較低的IgM,后期以IgG為主。初次應(yīng)答過(guò)程的血清中抗原特異性抗體的高峰濃度較低且維持時(shí)間較短。再次體液免疫應(yīng)答是指曾被某抗原免疫過(guò)的宿主再次接觸該抗原時(shí)血清中迅速出現(xiàn)抗原特異性抗體的過(guò)程,其延遲相僅為4-5天,血清抗體以IgG為主,其高峰濃度較高且維持時(shí)間較長(zhǎng)。再次應(yīng)答所產(chǎn)生的抗體還有親和力成熟現(xiàn)象,即該抗體與抗原的親和力明顯高于初次應(yīng)答產(chǎn)生的抗體。
溶質(zhì)載體家族蛋白34成員A3抗體,Anti-SLC34A3抗體相關(guān)產(chǎn)品:
HEV 戊型肝炎病毒抗體
HGF(hepatocyte growth factor) 肝細(xì)胞生長(zhǎng)因子抗體
HGV(Hepatitis G vivus) 庚型肝炎病毒抗體
ZEBRA/HHV4/EBV(Human herpesvirus 4 type 2/epstein-barr virus ) 類(lèi)皰疹病毒4抗體
HHV8/ORF K2(Human herpesvirus 8) 類(lèi)皰疹病毒8抗體
HHV8/ORF K2(Human herpesvirus 8) 類(lèi)皰疹病毒8抗體
HHV8/ORF50(Human herpesvirus 8 type P) 類(lèi)皰疹病毒8抗體
HHV8/ORF50(Human herpesvirus 8 type P) 類(lèi)皰疹病毒8抗體
HHV8/ORF K2(Human herpesvirus 8 type P) 類(lèi)皰疹病毒8抗體
HIF-1α (YMpoxia-inducible factor -1α) 缺氧誘導(dǎo)因子1α 抗體
HIF-1β/ARNT1(YMpoxia-inducible factor -1β) 缺氧誘導(dǎo)因子1β 抗體
HIF-2α (YMpoxia-inducible factor-2α) 缺氧誘導(dǎo)因子2α 抗體
HINT1(histidine triad nucleotide binding protein 1) *三聚體核苷結(jié)合蛋白1抗體
Histone H3,Family 3A (HISTIH3I protein) 抗組蛋白3抗體
Histone H1 抗組蛋白H1抗體
Histone H1b/Histone H1.4(Ac-Lys53) 抗乙酰化組蛋白H1b抗體
Histone H2/HTB901 抗組蛋白H2抗體
Histone H3-like protein 組蛋白H3樣抗體(Brassica juncea)
Histone H3-like protein 組蛋白H3樣抗體(Brassica junceaC端抗體)
Histone H3-like protein 組蛋白H3樣蛋白抗體(Brassica juncea N端抗體)
免疫組化操作步驟:
(一)、儀器設(shè)備
1、 18cm不銹鋼高壓鍋或電爐或用微波爐
2、 水浴鍋
(二)、試 劑
1、 PBS緩沖液(pH7.2―7.4)
2、 0.01mol/L檸檬酸鈉緩沖液(CB,pH6.0,1000ml)
3、 3%甲醇―H2O2溶液:用30% H2O2 和80%甲醇溶液配制
4、 風(fēng)裱劑:
a. 甘油和0.5mmol/L碳酸鹽緩沖液(pH9.0–9.5)等量混合
b. 油和TBS(PBS)配制
5、 TBS/PBS pH9.0–9.5,適用于熒光纖維鏡標(biāo)本;pH7.0-7.4適合光學(xué)纖維標(biāo)本
(三)、操作流程
1、 脫蠟和水化:脫蠟前應(yīng)將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘
a. 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b. 無(wú)水乙醇中浸泡五分鐘
c. 95%乙醇中浸泡五分鐘
d. 75%乙醇中浸泡五分鐘
2、 抗原修復(fù):用于福爾馬林固定的石蠟包埋組織芯片