中文名稱:兔抗小鼠IgA抗體
英文名稱:Anti-Mouse IgA antibody
上海研盟生物科技有限公司Anti-Mouse IgA抗體*,主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發送,您也可以直接我司在線客服索取??头?.
兔抗小鼠IgA抗體,Anti-Mouse IgA抗體簡單介紹:
產品別名:A2m marker; FLJ14473; FLJ35065; FLJ35500; FLJ36402; FLJ39698; FLJ40001; FLJ41548; FLJ41552; FLJ41789; FLJ43248; FLJ43594; FLJ44293; FLJ46028; FLJ46621; FLJ46724; FLJ46811; FLJ46824; Ig alpha 1 chain C region; Ig alpha 2 chain C region; IgA1; Igh2; IGHA 1; IGHA 2; IGHA1; IGHA2; Immunoglobulin alpha 1; Immunoglobulin Am1; Immunoglobulin Am2; Immunoglobulin heavy chain 2 (serum IgA); Immunoglobulin heavy constant alpha 1; Immunoglobulin heavy constant alpha 2; MGC102857.
產品規格:0.1ml、0.2ml、1ml
產品用途:科研實驗
產品價格:請報價
說明書:請添加客服直接索取
抗體來源:該指標有兩種產品,一種是兔來源抗體,一種是鼠來源抗體
克隆類型:兔來源為多克隆抗體,鼠來源單克隆抗體
交叉反應:請索取說明書查看
背景介紹:Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.
兔抗小鼠IgA抗體保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
免疫應答過程:
1.抗原進入機體;
2.抗原刺激 B淋巴細胞,使之分化增殖成為抗體分泌漿細胞和記憶細胞(初次免疫應答);
3.抗體主要由漿細胞分泌 ;
4.記憶細胞再次受到特異性抗原刺激后會被激活而成為分泌抗體的漿細胞(再次免疫應答);
5.記憶B細胞是長命細胞,可以存活數月甚至機體終生 。
初次體液免疫是指宿主*次接觸外來TD抗原后血清中逐漸出現抗原特異性抗體的過程。初次體液免疫應答的延遲相(從抗原免疫到抗體水平達到高峰的時間)為6-10天,起初幾天血清中的抗原特異性抗體主要為親和力較低的IgM,后期以IgG為主。初次應答過程的血清中抗原特異性抗體的高峰濃度較低且維持時間較短。再次體液免疫應答是指曾被某抗原免疫過的宿主再次接觸該抗原時血清中迅速出現抗原特異性抗體的過程,其延遲相僅為4-5天,血清抗體以IgG為主,其高峰濃度較高且維持時間較長。再次應答所產生的抗體還有親和力成熟現象,即該抗體與抗原的親和力明顯高于初次應答產生的抗體。
兔抗小鼠IgA抗體,Anti-Mouse IgA抗體相關產品:
HEV 戊型肝炎病毒抗體
HGF(hepatocyte growth factor) 肝細胞生長因子抗體
HGV(Hepatitis G vivus) 庚型肝炎病毒抗體
ZEBRA/HHV4/EBV(Human herpesvirus 4 type 2/epstein-barr virus ) 類皰疹病毒4抗體
HHV8/ORF K2(Human herpesvirus 8) 類皰疹病毒8抗體
HHV8/ORF K2(Human herpesvirus 8) 類皰疹病毒8抗體
HHV8/ORF50(Human herpesvirus 8 type P) 類皰疹病毒8抗體
HHV8/ORF50(Human herpesvirus 8 type P) 類皰疹病毒8抗體
HHV8/ORF K2(Human herpesvirus 8 type P) 類皰疹病毒8抗體
HIF-1α (YMpoxia-inducible factor -1α) 缺氧誘導因子1α 抗體
HIF-1β/ARNT1(YMpoxia-inducible factor -1β) 缺氧誘導因子1β 抗體
HIF-2α (YMpoxia-inducible factor-2α) 缺氧誘導因子2α 抗體
HINT1(histidine triad nucleotide binding protein 1) *三聚體核苷結合蛋白1抗體
Histone H3,Family 3A (HISTIH3I protein) 抗組蛋白3抗體
Histone H1 抗組蛋白H1抗體
Histone H1b/Histone H1.4(Ac-Lys53) 抗乙?;M蛋白H1b抗體
Histone H2/HTB901 抗組蛋白H2抗體
Histone H3-like protein 組蛋白H3樣抗體(Brassica juncea)
Histone H3-like protein 組蛋白H3樣抗體(Brassica junceaC端抗體)
Histone H3-like protein 組蛋白H3樣蛋白抗體(Brassica juncea N端抗體)
免疫組化操作步驟:
(一)、儀器設備
1、 18cm不銹鋼高壓鍋或電爐或用微波爐
2、 水浴鍋
(二)、試 劑
1、 PBS緩沖液(pH7.2―7.4)
2、 0.01mol/L檸檬酸鈉緩沖液(CB,pH6.0,1000ml)
3、 3%甲醇―H2O2溶液:用30% H2O2 和80%甲醇溶液配制
4、 風裱劑:
a. 甘油和0.5mmol/L碳酸鹽緩沖液(pH9.0–9.5)等量混合
b. 油和TBS(PBS)配制
5、 TBS/PBS pH9.0–9.5,適用于熒光纖維鏡標本;pH7.0-7.4適合光學纖維標本
(三)、操作流程
1、 脫蠟和水化:脫蠟前應將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘
a. 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b. 無水乙醇中浸泡五分鐘
c. 95%乙醇中浸泡五分鐘
d. 75%乙醇中浸泡五分鐘
2、 抗原修復:用于福爾馬林固定的石蠟包埋組織芯片