上海研盟生物有限公司*品質供應Anti-SREBP1抗體,我公司銷售國內外科研產品,如生物試劑、生化試劑,一抗二抗,各種標記抗體,Elisa酶聯免疫試劑盒,放免試劑盒,培養基,各種動物血清等產品,歡迎廣大新老客戶前來咨詢訂購,訂購:或。
*調節元件結合蛋白1抗體,Anti-SREBP1抗體簡單介紹:
【中文名稱】:*調節元件結合蛋白1抗體
【英文名稱】:Anti-SREBP1抗體
【英文別名】:Sterol regulatory element binding protein 1; ADD 1; BHLHD1; D630008H06; SREBF 1; SREBP-1; SREBF1; SREBP 1; SREBP1; SREBP 1c; Sterol regulatory element binding protein 1; Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1; Sterol regulatory element binding transcription factor 1; SRBP1_HUMAN; Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1; Processed sterol regulatory element-binding protein 1.
【規 格】:0.1ml/0.2ml
【相關標記】:Alexa Fluor 350、Alexa Fluor 488、Alexa Fluor 555、Alexa Fluor 647、AP、APC、Biotin、Cy3、Cy5、Cy5.5、Cy7、FITC、Gold、HRP、PE、PE-Cy3、PE-CY5、PE-CY5.5、PE-CY7、RBITC.【濃 度】:1mg/1ml
【抗體來源】:Rabbit or Mouse or Goat
【克隆類型】:polyclonal or monoclonal
【產品類型】:一抗
【產品應用】:可以用于做石蠟切片免疫組化,冰凍切片免疫組化,Elisa,WB,免疫熒光、流式細胞術等實驗。
*調節元件結合蛋白1抗體【背景介紹】:This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene.
*調節元件結合蛋白1抗體,Anti-SREBP1抗體免疫組化操作流程:
(一) 儀器設備
1. 18cm不銹鋼高壓鍋或電爐或用微波爐.
2. 水浴鍋
(二) 試 劑
1. PBS緩沖液(ph7.2―7.4):NaC137mmol/L,KCl 2.7mmol/L ,Na2HPO4 4.3mmol/L,
KH2PO4 1.4mmol/L.
2.0.01mol/L檸檬酸鈉緩沖液(CB,ph6.0,1000ml):檸檬酸三鈉3g,檸檬酸0.4g。
3.0.5mol/L EDTA緩沖液(ph8.0):700ml水中溶解186.1g EDTA&
8226;2H2O,用10mmol/L
NaOH調至ph8.0,加水至1000ml.
4. 1mol/L的TBS緩沖液(ph8.0):在800ml水中溶解121gTris堿,用1N的HCl調至ph8.0,加水1000ml。
5. 酶消化液:a. 0.1%*:用0.1%CaCl 12(ph7.8)配制。b.0.4%*液:用0.1N的HCl配制。
6. 3%甲醇―H2O2溶液:用30%H2O2和80%甲醇溶液配制
7. 風裱劑:a. 甘油和0.5mmol/L碳酸鹽緩沖液(ph9.0–9.5)等量混合
b 油和TBS(PBS)配制
8.TBS/PBS PH9.0–9.5,適用于熒光纖維鏡標本;ph7.0-7.4適合光學纖維標本。
(三) 操作流程
1 脫蠟和水化:脫蠟前應將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘。 a 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b 無水乙醇中浸泡五分鐘
c 95%乙醇中浸泡五分鐘
d 75%乙醇中浸泡五分鐘
2 抗原修復:用于福爾馬林固定的石蠟包埋組織芯片
★研盟生物 ★合作: ★客服
相關標記抗體:HRP標記抗體,Biotin標記抗體,Gold標記抗體,RBITC標記抗體,AP標記抗體,FITC標記抗體,Cy3標記抗體,Cy5標記抗體,Cy5.5標記抗體,Cy7標記抗體,PE標記抗體,PE-Cy3標記抗體,PE-Cy5標記抗體,PE-Cy5.5標記抗體,PE-Cy7標記抗體,APC標記抗體,Alexa Fluor 350標記抗體,Alexa Fluor 488標記抗體,Alexa Fluor 555標記抗體,Alexa Fluor 647標記抗體
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