上海研盟生物科技有限公司Anti-RhD抗體*,主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發送,您也可以直接我司在線客服索取。客服
英文名稱:Anti-RhD antibody
中文名稱:Rh polypeptide 2抗體
Rh polypeptide 2抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Rh polypeptide 2抗體,Anti-RhD抗體抗原的分類:
一. 免疫學分類:
(1)*抗原、半抗原;
(2)胸腺依賴抗原與非依賴抗原。
二. 臨床分類:
(1)外源性抗原:微生物、花粉等;
(2)內源性抗原:隱蔽的自身抗原、腫瘤相關抗原等;
(3)同種異型抗原:人類白細胞抗原、血型抗原;
(4)異嗜性抗原:人與其他動物、植物等之間存在的共同抗原。
★研盟生物★Rh polypeptide 2抗體,Anti-RhD抗體
Western Blotting 實驗步驟概述:
1、 組織取材:組織塊稱重
2、 利用液氮、研缽粉碎組織塊
3、 加入RIPA緩沖液(每克組織3ml RIPA),PMSF(每克組織30μl,10 mg/ml PMSF),
利用Polytron進一步勻漿(15,000轉/分*1分鐘)維持4℃
4、 加入PMSF(每克組織30μl,10 mg/ml PMSF),冰上孵育30分鐘
5、 移入離心管4℃,約20,000g(約15,000轉)15分鐘
6、 上清液為細胞裂解液可分裝-20℃保存
7、 進行Bradford比色法測定蛋白質濃度
8、 取相同質量的細胞裂解液(體積*蛋白質濃度),并加等體積的2×電泳加樣緩沖液
9、 沸水浴中3分鐘
10、上樣
11、電泳(濃縮膠20mA,分離膠35mA)
12、電轉膜儀轉膜(100mA 40分鐘)
13、膜用麗春紅染色,膠用考馬斯亮藍染色
14、Westernblot 試劑盒顯色
15、分析比較記錄
★研盟生物 ★合作: ★客服
相關標記抗體:HRP標記抗體,Biotin標記抗體,Gold標記抗體,RBITC標記抗體,AP標記抗體,FITC標記抗體,Cy3標記抗體,Cy5標記抗體,Cy5.5標記抗體,Cy7標記抗體,PE標記抗體,PE-Cy3標記抗體,PE-Cy5標記抗體,PE-Cy5.5標記抗體,PE-Cy7標記抗體,APC標記抗體,Alexa Fluor 350標記抗體,Alexa Fluor 488標記抗體,Alexa Fluor 555標記抗體,Alexa Fluor 647標記抗體
簡單介紹:
產品別名:Blood group Rh(D) polypeptide; Blood group--Rhesus system D polypeptide; CD240D; D antigen (DCS); DIIIc; MGC165007; RH; Rh blood group antigen Evans; Rh blood group D antigen; Rh polypeptide 2; RH30; Rh4; RHCED; Rhd; RHD_HUMAN; RhDCw; RHDel; RHDVA(TT); Rhesus blood group D antigen allele DIII type 7; Rhesus D antigen; Rhesus system D polypeptide; RhII; RhK562-II; RhPI.
抗體來源:Rabbit or Mouse
保質期:1年
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene, which encodes the RhD protein, and a second gene that encodes both the RhC and RhE antigens on a single polypeptide. The two genes, and a third unrelated gene, are found in a cluster on chromosome 1. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. Multiple transcript variants encoding different isoforms have been found for this gene.
相關優質產品:
Cdkn1c/p57/Kip2 (Cyclindependent kinase inhibitor 1C) 周期蛋白依賴激酶抑制因子1C抗體
CGP-39/YKL-40(Chitinase-3-like protein 1 ) 軟骨糖蛋白39抗體
CEA (Carcinoembryonic Antigen ) 癌胚抗原抗體
CEA(mouse monoclonal) 鼠抗癌胚抗原單克隆抗體(包被)
CEA(mouse monoclonal) 鼠抗癌胚抗原單克隆抗體(優質產品)
CEAcam8/CD67 (carcinoembryonic gen-related cell adhesion molecule 8) 癌胚抗原相關細胞粘附分子8抗體
CEBP-α /CLEBPα (CCAAT Enhancer Binding Protein alpha) 轉錄調節因子C/EBP α 抗體
CEBP β(CCAAT enhancer binding protein(CEBP),beta) 轉錄調節因子C/EBP β抗體
Cecropin 抗菌肽/天蠶素抗體
C-erbB-2(HER2/NEU) c-erbB-2(HER2/NEU)抗體
C-erbB-4/HER4/erbB-4 (epidermal growth factor receptor4) c-erbB-4癌基因抗體
c-fos 即刻早期基因(原癌基因)抗體
CFTR(cystic fibrosis transmembrane conductance regulator) 囊性纖維化跨膜轉運調節因子抗體
CGA(Chromogranin A) 嗜鉻粒素A抗體
CGB(Chromogranin B) 嗜鉻粒素B抗體
CGRP (calcitonin gene related peptide) 降鈣素基因相關肽抗體
ChAT(Choline Acetyltransferase) *乙酰轉移酶抗體
CHK1(Checkpoint kinase 1) 抗細胞周期優質產品點激酶1抗體
CHK2(Checkpoint kinase 2) 細胞周期優質產品點激酶2抗體