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上海研盟生物科技有限公司
上海研盟生物科技有限公司“Anti-Laminin Beta 1抗體,層粘連蛋白β1抗體"抗體規格0.1ml、0.2ml、1ml,抗體產品有效期一年,質保三個月,質保期內出現任何質量問題或是出不了實驗結果均可免費退換。:、
上海研盟生物科技有限公司Anti-Laminin Beta 1抗體,層粘連蛋白β1抗體*,主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發送,您也可以直接我司在線客服索取。:
中文名稱:層粘連蛋白β1抗體
英文名稱:Anti-Laminin Beta 1抗體
產品編號:BYK-1526R
別名:LAMA 1 LAMA; LAMA1; LAMB1; CLM; Cutis laxa with marfanoid phenotype; LAM B1; LAMB 1; LAMB1_HUMAN; Laminin B1; Laminin beta 1 chain; Laminin beta 1 chain precursor; Laminin beta1; Laminin subunit beta-1; Laminin-1 subunit beta; Laminin-10 subunit beta; Laminin-12 subunit beta; Laminin-2 subunit beta; Laminin-6 subunit beta; Laminin-8 subunit beta; MGC142015; Laminin β1;Laminin A chain; Laminin alpha 1; Laminin alpha 1 chain; Laminin B1 chain; Laminin subunit beta 1.
此產品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
規格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來源:本公司抗體有兔來源和鼠來源等
克隆類型:兔來源為多抗,鼠來源為單抗
產品類型:一抗
Anti-Laminin Beta 1抗體,層粘連蛋白β1抗體產品介紹:Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm(EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis.
上海研盟生物科技有限公司Western Blotting 問題解答
1、膠片上存在反轉印象(如:亮的條帶、黑的背景)?
原因:HRP的濃度過高;
解決:稀釋HRP至少10倍。
2、膜上有黃色或棕色的沉積?
原因:HRP的濃度過高;
解決:稀釋HRP至少10倍。
3、在暗室中條帶過于明亮?
原因:HRP的濃度過高;
解決:稀釋HRP至少10倍。
4、條帶的發光時間持續了至少8小時?
原因:HRP的濃度過高;
解決:稀釋HRP至少10倍。
5、信號太弱或沒有?
原因:
1.太多的HRP積聚導致信號快速的消退;
2.抗原或抗體的濃度不夠;
3.轉印不成功;
4.HRP或其他底物的效價降低。
解決:
1.稀釋HRP至少10倍;
2.增加其濃度;
3.尋找合適的轉移方法和條件;
4.選擇其他的合適底物。
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