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上海研盟生物科技有限公司
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更新時(shí)間：2017-11-13 18:28:49瀏覽次數(shù)：202次
聯(lián)系我時(shí)，請(qǐng)告知來自 環(huán)保在線“Anti-ATF1抗體,活化轉(zhuǎn)錄因子1抗體“抗體規(guī)格0.1ml、0.2ml、1ml，抗體產(chǎn)品有效期一年，質(zhì)保三個(gè)月，質(zhì)保期內(nèi)出現(xiàn)任何質(zhì)量問題或是出不了實(shí)驗(yàn)結(jié)果均可免費(fèi)退換：
上海研盟生物科技有限公司Anti-ATF1抗體,活化轉(zhuǎn)錄因子1抗體*，主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送，您也可以直接我司在線客服索取。客服
簡(jiǎn)單介紹：
中文名稱：活化轉(zhuǎn)錄因子1抗體
英文名稱：Anti-ATF1
產(chǎn)品編號(hào)：byk-0517R
產(chǎn)品別名：Activating Transcription Factor1; TREB36 protein; ATF 1; ATF1 EWS fusion gene ATF1 FUS fusion gene; cAMP dependent transcription factor 1; Cyclic AMP dependent transcription factor ATF 1; Cyclic AMP dependent transcription factor ATF1; EWS AFT1; FUS ATF 1; RNA binding protein activating transcription factor 1 fusion protein; TREB 36; TREB36; ATF1_HUMAN.
產(chǎn)品用途：科研實(shí)驗(yàn)
產(chǎn)品價(jià)格：請(qǐng)報(bào)價(jià)
說明書：請(qǐng)?zhí)砑涌头苯铀魅?/p>
抗體來源：該指標(biāo)有兩種產(chǎn)品，一種是兔來源抗體，一種是鼠來源抗體
克隆類型：兔來源為多克隆抗體，鼠來源單克隆抗體
交叉反應(yīng)：請(qǐng)索取說明書查看
性 狀：Lyophilized or Liquid
濃 度：1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲(chǔ) 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-ATF1抗體,活化轉(zhuǎn)錄因子1抗體產(chǎn)品介紹：ATF1 (Activating Transcription Factor 1, TREB-36) is a member of the ATF/CREB family of basic region leucine-zipper (bZip) DNA-binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. The activation function of CRE-binding proteins may be modulated by phosphorylation of several kinases and is mediated by coactivators such as CREB-binding protein (CBP) and p300. ATF1 is a nuclear protein that binds DNA as a homodimer or as heterodimers with the inducible transcription factors CREB1 or CREM. Heterodimers appear to be stronger transcriptional activators than the homodimers. Tissue expression of ATF1 mRNA is widespread. Several isoforms of ATF1 arise by differential splicing. ATF1 mediates both Ca2+ and cAMP responses at several levels. It binds to the Tax-responsive element (TRE1) of the human T-cell lymphotropic virus type-I (HTLV1). ATF1 is detectable in metastatic melanoma cells and seems to contribute to their survival. A chimeric protein composed of the N-terminal domain of EWS (Ewing sarcoma oncogene) linked to the bZip domain of ATF1 is implicated in the rare malignant clear cell sarcoma of tendon sheath and aponeuroses (malignant melanoma of soft parts).
免疫組化的呈色深淺可反映抗原存在的數(shù)量，可作為定性、定位和定量的依據(jù)。
1.陽性細(xì)胞染色分布有三種類型：細(xì)胞漿、細(xì)胞核、細(xì)胞膜表面。大部分抗原見于細(xì)胞漿，可見于整個(gè)胞漿或部分胞漿
2.陽性細(xì)胞分布可分為灶型和彌漫性
3.由于細(xì)胞內(nèi)含抗原量不同，所以染色強(qiáng)度不一。如果細(xì)胞之間染色強(qiáng)度相同，常提示其反應(yīng)為非特異性
4.陽性細(xì)胞染色定位于單個(gè)細(xì)胞，且與陰性細(xì)胞相互交雜分布；而非特異性染色常不限于單個(gè)細(xì)胞，而是累及一片細(xì)胞。
5.切片邊緣、刀痕或褶皺區(qū)域，壞死或擠壓的細(xì)胞區(qū)，膠原結(jié)締組織等，常表現(xiàn)為相同的陽性染色強(qiáng)度，不能用于判斷陽性。
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