當前位置：賽默飛世爾科技（中國）有限公司集團>>Western 免疫印跡產品>>抗體結合蛋白和偶聯物>>Pierce™ 重組蛋白 A/G

Pierce™ 重組蛋白 A/G

返回列表頁

參考價

面議

具體成交價以合同協議為準

產品型號Thermo Scientific

品牌

廠商性質0

所在地

在線詢價收藏產品查看聯系電話

聯系方式：客服查看聯系方式

更新時間：2023-11-06 15:45:51瀏覽次數：144次

聯系我時，請告知來自環保在線

產品分類 品牌分類

  Western 免疫印跡產品

Western 印跡設備和耗材

Western 印跡和核酸膜及濾紙

通用 Western 印跡試劑

抗體結合蛋白和偶聯物

Western 印跡底物和底物試劑盒

蛋白方法封閉緩沖液

靶標特異性 Western 印跡試劑盒

X 射線膜和耗材

結合蛋白和偶聯物

全部產品列表

暫無信息

賽默飛世爾科技（中國）有限公司集團

經營模式：

商鋪產品：2508條

所在地區：

聯系人：客服（客服）

詢價 給他留言

產品簡介

Pierce™ 重組蛋白 A/G

詳細介紹

Purified (unconjugated) Thermo Scientific Pierce Recombinant Protein A/G is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

Features of Recombinant Protein A/G:

• Contains four Fc-binding domains from Protein A and two from Protein G
• Provides binding for all antibody species and subclasses recognized by either Protein A or Protein G

The extended Fc-binding properties of Protein A/G make it a popular tool in the investigation and purification of immunoglobulins. Protein A/G binds to all human IgG subclasses, IgA, IgE, IgM and to a lesser extent IgD; however, it does not bind mouse IgA, IgM or murine serum albumin. Protein A/G is an excellent tool for purification and detection of mouse monoclonal antibodies from IgG subclasses without interference from these other serum proteins. Individual subclasses of mouse monoclonals are most likely to have stronger affinity to this chimeric protein than to either Protein A or Protein G.

Properties of Recombinant Protein A/G:
• Source: E. coli
• Molecular weight: 50,460 (Apparent MW by SDS-PAGE: 40,000-45,000)
• Form: salt-free powder
• A280 of 0.1% solution: ∼0.505
• Isoelectric point (pI): 4.65

Gene fusion of the Fc-binding domains of Protein A and Protein G has resulted in production of a structurally and functionally chimeric protein with broader binding than either Protein A or Protein G alone. During fusion, the Protein G gene sequence coding for the serum albumin-binding site is eliminated. This protein is expressed in E. coli and secreted into the surrounding medium during fermentation. The product obtained is consistent in quality and yield because the bacterial host is engineered to be deficient in major proteolytic activities. Binding is less pH-dependent than either Protein A or Protein G alone, occurring well at pH 5 to 8.

Related Products
Pierce™ Recombinant Protein A/G, Alkaline Phosphatase Conjugated
Pierce™ Recombinant Protein A/G, Peroxidase Conjugated

For Research Use Only. Not for use in diagnostic procedures.