小鼠纖溶抑制因子(TAFI)ELISA檢測試劑盒
英文名稱：mouse Thrombin Activatable Fibrinolysis Inhibitor (TAFI) ELISA Kit
貨號：ZK-M5216
規格： 96T/48T
產品包裝：盒（KIT）（含試劑+酶聯板+覆膜等）
適用范圍：實驗結果僅供科研參考，不推薦用于臨床診斷結果。
有效期： 4 個月(4℃)；8 個月（-20℃）。 

小鼠纖溶抑制因子(TAFI)ELISA檢測試劑盒Assay procedure
1.  Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.
2.  Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.
3.  Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.
4.  Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 
5.  Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.
6.  Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.
7.  Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not 
appear uniform, gently tap the plate to ensure thorough mixing.
8.  Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.

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