牛纖維蛋白原(FG) ELISA試劑盒子科生物現貨供應,要想選對合適品牌的ELISA試劑盒,請您放心購買我司ZIKER子科生物品牌試劑盒,高靈敏度、重復性,強特異性、操作性,值得您的選擇,讓您有一個滿意的實驗結果!
牛纖維蛋白原(FG) ELISA試劑盒?品牌:子科生物ZIKER,美國R&D,美國immonoway,美國sciencell,德國IBL
?產品說明書:可添加在線客服索要原版詳細操作說明書,或者留下您的,我們會將產品詳細說明書發送至您的
?價格:電議,或咨詢在線客服,或者以郵件形式發到我司zikerbio進行詢價,我們將在*時間回復您;深圳子科生物提供大量品牌進口原裝,分裝,以及穩定性強的國產ELISA試劑盒,性價比*。
牛纖維蛋白原(FG) ELISA試劑盒組成試劑盒:牛纖維蛋白原(FG) ELISA試劑盒實驗測定具體步驟:
1.確定本次檢測所需的已包被抗體的酶標板孔數目,并增加 1 孔作為 TMB 空白顯色孔。 總數=樣品數+9;做雙份檢測時×2。其余重包裝好放如冰箱中。
2.將標準品各 0.1ml 依次加入一排 7 孔中,1 孔只加樣品稀釋液的作為零孔。對于血清、體液、 組織勻漿或細胞培養上清,直接加已用樣品稀釋液稀釋的樣品 100μ ι 。
3.酶標板加上蓋,37℃反應 90 分鐘。
4.反應后用自動洗板機吸去酶標板內的液體;或甩去酶標板內液體,再對著吸水紙拍幾下。 不洗。
5.將準備好的生物素抗體工作液按每孔 0.1ml 依次加入。(TMB 空白顯色孔除 外)。37℃反應 60 分鐘。
6. 0.01M TBS 或 0.01M PBS 洗滌 3 次,每次浸泡 1 分鐘左右。
7.將準備好的 ABC 工作液按每孔 0.1ml 依次加入(TMB 空白顯色孔除外)。37℃反應 30 分鐘。
8. 0.01M TBS 或 0.01M PBS 洗滌 5 次,每次浸泡 1-2 分鐘左右。
9.按每孔 90μ ι 依次加入已在 37℃平衡 30 分鐘的 TMB 顯色液,37℃避光反應 20-25 分鐘 (注意:顯色時間供參考,因用戶實驗室條件差異,顯色時間會有所不同。此時肉眼可 見標準品的前 3-4 孔有明顯的梯度藍色,后 3-4 孔差別不明顯)。
10.按每孔 0.1ml 依次加入 TMB 終止液,此時藍色立轉黃色。
11.用酶標儀在 450nm 測定 OD值。
牛纖維蛋白原(FG) ELISA試劑盒相關產品如下:RC-547 5-Bromo-4-chloro-3-indoxyl-alpha-D-glucopyranoside 2.5 G 12 MONTHS
RC-548 5-Bromo-4-chloro-3-indoxyl-alpha-D-glucopyranoside 25 G 12 MONTHS
RC-549 5-Bromo-4-chloro-3-indoxyl-alpha-D-glucopyranoside 0.25 G 12 MONTHS
RC-550 5-Bromo-4-chloro-3-indoxyl-alpha-D-glucopyranoside 5 G 12 MONTHS
RC-551 5-Bromo-4-chloro-3-indoxyl-alpha-D-glucopyranoside 50 G 12 MONTHS
RC-552 5-Bromo-4-chloro-3-indoxyl-alpha-D-glucopyranoside 0.5 G 12 MONTHS
RC-553 5-Bromo-4-chloro-3-indoxyl-alpha-D-mannopyranoside 1 G 12 MONTHS
RC-554 5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, sodium salt 1 G 12 MONTHS
RC-555 5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, sodium salt 0.01 G 12 MONTHS
RC-556 5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, sodium salt 0.1 G 12 MONTHS
RC-557 5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, sodium salt 0.025 G 12 MONTHS
RC-558 5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, sodium salt 0.25 G 12 MONTHS
RC-559 5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, sodium salt 0.05 G 12 MONTHS
RC-560 5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, sodium salt 0.5 G 12 MONTHS
RC-561 5-Bromo-4-chloro-3-indoxyl-alpha-D-xylopyranoside 0.5 G 12 MONTHS
RC-562 5-Bromo-4-chloro-3-indoxyl-alpha-L-arabinofuranoside 0.1 G 12 MONTHS
RC-563 5-Bromo-4-chloro-3-indoxyl-alpha-L-arabinofuranoside 0.025 G 12 MONTHS
RC-564 5-Bromo-4-chloro-3-indoxyl-alpha-L-fucopyranoside 0.1 g 12 MONTHS
RC-565 5-Bromo-4-chloro-3-indoxyl-alpha-L-fucopyranoside 0.25 g 12 MONTHS
RC-566 5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside 1 G 12 MONTHS
RC-567 5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside 10 G 12 MONTHS
RC-568 5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside 2.5 G 12 MONTHS
RC-569 5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside 25 G 12 MONTHS
RC-570 5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside 0.25 G 12 MONTHS
RC-571 5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside 5 G 12 MONTHS
RC-572 5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside 0.5 G 12 MONTHS
RC-573 5-Bromo-4-chloro-3-indoxyl-beta-D-fucopyranoside 1 G 12 MONTHS
RC-574 5-Bromo-4-chloro-3-indoxyl-beta-D-fucopyranoside 0.1 G 12 MONTHS
RC-575 5-Bromo-4-chloro-3-indoxyl-beta-D-fucopyranoside 0.25 G 12 MONTHS
RC-576 5-Bromo-4-chloro-3-indoxyl-beta-D-fucopyranoside 0.05 G 12 MONTHS
RC-577 5-Bromo-4-chloro-3-indoxyl-beta-D-fucopyranoside 0.5 G 12 MONTHS
RC-578 5-Bromo-4-chloro-3-indoxyl-beta-D-glucopyranoside 1 G 12 MONTHS
RC-579 5-Bromo-4-chloro-3-indoxyl-beta-D-glucopyranoside 10 G 12 MONTHS
RC-580 5-Bromo-4-chloro-3-indoxyl-beta-D-glucopyranoside 100 G 12 MONTHS
RC-581 5-Bromo-4-chloro-3-indoxyl-beta-D-glucopyranoside 2.5 G 12 MONTHS
RC-582 5-Bromo-4-chloro-3-indoxyl-beta-D-glucopyranoside 25 G 12 MONTHS
RC-583 5-Bromo-4-chloro-3-indoxyl-beta-D-glucopyranoside 250 G 12 MONTHS
RC-584 5-Bromo-4-chloro-3-indoxyl-beta-D-glucopyranoside 5 G 12 MONTHS
RC-585 5-Bromo-4-chloro-3-indoxyl-beta-D-glucopyranoside 50 G 12 MONTHS
RC-586 5-Bromo-4-chloro-3-indoxyl-beta-D-glucopyranoside 0.5 G 12 MONTHS
RC-587 5-Bromo-4-chloro-3-indoxyl-beta-D-glucopyranoside 500 G 12 MONTHS
RC-588 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt trihydrate 1 G 12 MONTHS
RC-589 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt trihydrate 10 G 12 MONTHS
RC-590 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt trihydrate 100 G 12 MONTHS
RC-591 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt trihydrate 2.5 G 12 MONTHS