小鼠葡萄糖依賴性胰島素釋放多肽(GIP)ELISA試劑盒子科生物現貨供應,子科生物進口elisa試劑盒,國產elisa試劑盒,elisa試劑盒說明書,elisa試劑盒免費代測,ELISA試劑盒技術服務,如需Elisa試劑盒說明書及報價,可我司。
●產品名稱:小鼠葡萄糖依賴性胰島素釋放多肽(GIP)ELISA試劑盒
●英文名稱:Mouse Glucose-dependent insulin-releasing polypeptide,GIP ELISA Kit
●貨號:ZK-M5092
●規格: 96T/48T
●品牌:子科生物ZIKER
●反應時間: 1-5h
●所需樣本體積: 50-100ul
●檢測波長: 450 nm
●用途: For research use only. Not for diagnostic use.
●特點:靈敏性高、特異性強、重復性好
●服務:子科生物所有的elisa試劑盒均可免費代測,全程Elisa實驗技術指導。
●運輸:全國各地快遞免費送貨上門,低溫運輸,用冰袋加泡沫保溫盒包裝,確保產品運輸狀態下也處于低溫狀態。
凡購買我們公司的ELISA相關系列產品,都可以享受免費代測服務,只收取試劑盒的相關費用,實驗檢測和實驗涉及到的試劑全部都是免費提供,并可以提供zui終實驗數據分析工作。需要代測服務的客戶,您只需要準備收集好您的樣本,郵寄到我公司實驗室,本市的銷售人員可以上門取樣本,我公司收到樣本之后正常是在一周內給您檢測報告結果。
小鼠葡萄糖依賴性胰島素釋放多肽(GIP)ELISA試劑盒Assay procedure
1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.
2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.
3. Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.
4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C.
5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.
6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.
7. Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not
appear uniform, gently tap the plate to ensure thorough mixing.
8. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.
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