小鼠載脂蛋白H(Apo-H)ELISA試劑盒子科生物現(xiàn)貨供應(yīng),子科生物進(jìn)口elisa試劑盒,國產(chǎn)elisa試劑盒,elisa試劑盒說明書,elisa試劑盒免費(fèi)代測,ELISA試劑盒技術(shù)服務(wù),如需Elisa試劑盒說明書及報(bào)價(jià),可我司。
小鼠載脂蛋白H(Apo-H)ELISA試劑盒?檢測方法:酶聯(lián)免疫分析ELISA方法
?有效期: 4 個(gè)月(4℃);8 個(gè)月(-20℃)。
?保存方法: 2-8℃(頻繁使用時(shí)); -20℃(長時(shí)間不用時(shí))。
?品牌:子科生物ZIKER,美國R&D,美國immonoway,美國sciencell,德國IBL
?質(zhì)量保證:我告訴所有產(chǎn)品均需要經(jīng)過質(zhì)檢通過后,才允許出庫!
?適用范圍:實(shí)驗(yàn)結(jié)果僅供科研參考,不推薦用于臨床診斷結(jié)果。
?ELISA常用的方法:雙抗體夾心法、間接法、競爭法以及BAS-ELISA等,子科生物ELISA產(chǎn)品主要用雙抗體夾心法。
深圳子科生物所有ELISA試劑盒均需要經(jīng)過質(zhì)檢合格后才允許出庫,能有效控制產(chǎn)品質(zhì)量,廣大客戶可以放心購買。且子科生物所有ELISA試劑盒里的抗體均采用進(jìn)口品牌抗體,能有效保證產(chǎn)品的高效靈敏性,且我公司研發(fā)人員有豐富的研發(fā)經(jīng)驗(yàn),*的儀器設(shè)備,*的實(shí)驗(yàn)技術(shù),讓我公司產(chǎn)品優(yōu)于同等廠家系列產(chǎn)品,是您的*廠家。
小鼠載脂蛋白H(Apo-H)ELISA試劑盒注意:客戶收到我公司產(chǎn)品請*時(shí)間確認(rèn)試劑盒是否有破損,酶標(biāo)板是否封閉,使用前 TMB顯色液應(yīng)為無色透明溶液,若發(fā)現(xiàn)顏色異常,或者其他問題請及時(shí)與我司售后人員進(jìn)行,我們會*次安排進(jìn)行退換。
封板膜只限一次性使用,以避免交叉污染,底物請避光保存。使用之后所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理,以免造成污染!
用于標(biāo)記ELISA試劑盒里抗體或抗抗體的酶須具有下列特性:
1、有高度的活性和敏感性;
2、在室溫下穩(wěn)定;
3、反應(yīng)產(chǎn)物易于顯現(xiàn);
4、能商品化生產(chǎn)。
當(dāng)前應(yīng)用較多的有辣根過氧化物酶(HRP)、堿性磷酸酶、葡萄糖氧化酶等,其中以HRP應(yīng)用zui廣。
結(jié)果判定常用的幾種方法
1) 目測定性法:加入底物經(jīng)酶解和終止反應(yīng)后,肉眼觀察陽性孔顏色明顯深于(競爭法則淺于)陰性對照;與陰性對照的顏色接近者,判定為陰性。
2) 以樣品孔的OD值大于陰性對照OD值+2~3SD,作為陽性結(jié)果的閾值。
3) 以P/N值(陽性孔OD值/陰性孔OD值)大于或等于2.1為陽性;P/N值小于2.1,但大于1.5為可疑;P/N小于1.5為陰性。
定量測定結(jié)果根據(jù)標(biāo)準(zhǔn)曲線計(jì)算樣品中待測物的含量。
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