小鼠5羥色胺(5-HT)ELISA試劑盒子科生物現貨供應,凡購買子科生物子科生物任何一款ELISA酶聯免疫分析檢測試劑盒,都可以享受免費代測服務,外地客戶可以由技術老師的指導下正確放置好樣本的保存方式后快遞郵寄到我公司技術部,本地客戶可以享受免費上門取樣服務!
●產品名稱:小鼠5羥色胺(5-HT)ELISA試劑盒
●英文名稱:Mouse 5-Hydroxytryptamine,5HT ELISA Kit
●貨號:ZK-M4927
●規格: 96T/48T
●品牌:子科生物ZIKER
●反應時間: 1-5h
●所需樣本體積: 50-100ul
●檢測波長: 450 nm
●用途: For research use only. Not for diagnostic use.
●特點:靈敏性高、特異性強、重復性好
●服務:子科生物所有的elisa試劑盒均可免費代測,全程Elisa實驗技術指導。
●運輸:全國各地快遞免費送貨上門,低溫運輸,用冰袋加泡沫保溫盒包裝,確保產品運輸狀態下也處于低溫狀態。
深圳子科生物所有ELISA試劑盒均需要經過質檢合格后才允許出庫,能有效控制產品質量,廣大客戶可以放心購買。且子科生物所有ELISA試劑盒里的抗體均采用進口品牌抗體,能有效保證產品的高效靈敏性,且我公司研發人員有豐富的研發經驗,*的儀器設備,*的實驗技術,讓我公司產品優于同等廠家系列產品,是您的*廠家。
小鼠5羥色胺(5-HT)ELISA試劑盒實驗原理
用純化的抗體包被微孔板,制成固相載體,往包被抗該指標抗體的微孔中依次加入標本或標準品、生物素化的抗該指標抗體、HRP標記的親和素,經過*洗滌后用底物TMB顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成zui終的黃色。顏色的深淺和樣品中的該指標呈正相關。用酶標儀在450nm波長下測定吸光度(OD值),計算樣品濃度。
標本的采集及保存
1. 血清:全血標本請于室溫放置2小時或4℃過了夜后于1000 x g離心20分鐘,取上清即可檢測,或將標本放于-20℃或-80℃保存,但應避免反復凍融。
2.血漿:可用EDTA或肝素作為抗凝劑,標本采集后30分鐘內于2 - 8° C 1000 x g離心15分鐘,或將標本放于-20℃或-80℃保存,但應避免反復凍融。
3.細胞培養物上清或其它生物標本:1000 x g離心20分鐘,取上清即可檢測,或將標本放于-20℃或-80℃保存,但應避免反復凍融。
注:標本溶血會影響zui后檢測結果,因此溶血標本不宜進行此項檢測。
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