小鼠腫瘤壞死因子β(TNF-β)ELISA試劑盒子科生物現貨供應,要想選對合適品牌的ELISA試劑盒,請您放心購買我司ZIKER子科生物品牌試劑盒,高靈敏度、重復性,強特異性、操作性,值得您的選擇,讓您有一個滿意的實驗結果!
?產品名稱:小鼠腫瘤壞死因子β(TNF-β)ELISA試劑盒
?英文名稱:Mouse Tumor necrosis factor β,TNF-β ELISA KIT
?產品規格:96T(人份)/48T(人份)(單孔檢測分別可以檢測90份樣本,42分樣本,一般都需要留6個孔做標準曲線)。
?檢測方法:酶聯免疫分析ELISA方法
?有效期: 4 個月(4℃);8 個月(-20℃)。
?保存方法: 2-8℃(頻繁使用時); -20℃(長時間不用時)。
?品牌:子科生物ZIKER,美國R&D,美國immonoway,美國sciencell,德國IBL
?質量保證:我告訴所有產品均需要經過質檢通過后,才允許出庫!
?適用范圍:實驗結果僅供科研參考,不推薦用于臨床診斷結果。
?ELISA常用的方法:雙抗體夾心法、間接法、競爭法以及BAS-ELISA等,子科生物ELISA產品主要用雙抗體夾心法。
深圳子科生物所有ELISA試劑盒均需要經過質檢合格后才允許出庫,能有效控制產品質量,廣大客戶可以放心購買。且子科生物所有ELISA試劑盒里的抗體均采用進口品牌抗體,能有效保證產品的高效靈敏性,且我公司研發人員有豐富的研發經驗,*的儀器設備,*的實驗技術,讓我公司產品優于同等廠家系列產品,是您的*廠家。
深圳子科生物科技有限公司專業研發生產酶聯免疫ELISA試劑盒,ELISA 檢測的目的是為實驗提供準確可靠的定量分析實驗依據,為了保證實驗數據的可靠性,在實驗過程中必須堅持全面質量控制和全過程質量控制。在收集標本前都必須有一個完整的計劃。
子科生物技術部總結介紹如下:
小鼠腫瘤壞死因子β(TNF-β)ELISA試劑盒注意事項:
●每個標本量收集體積= 100ul × 檢測種類,如要做復孔,標本量收集體積= 100ul × 檢測 種類×2 。
● 對于作某一個具體指標來說,先用一系列稀釋度作一批標本測定,得出對實驗有意義 的一個稀釋度(即測定劑量反應曲線),然后用一個zui適稀釋度測定即可。
● 收集標本前必須清楚要檢測的成份是否足夠穩定。對收集后當天進行檢測的標本,儲存在 4 ℃ 備用,如有特殊原因需要周期收集標本,將標本及時分裝后放在 -20 ℃ 或 -70 ℃ 條 件下保存。避免反復凍融。標本 2 -8 ℃ 可保存 48 小時, -20 ℃ 可保存 1 個月。 -70 度 可保存 6 個月。部分激素類標本需添加抑肽酶。
● 血清標本采集時,應注意避免溶血,紅細胞溶解時會釋放出具有過氧化物酶活性的物質, 以 HRP 為標記的 ELISA 測定中,溶血標本可能會增加非特異性顯色。
●為了保證尿液檢測結果的準確性,必須正確收集尿液標本和保存。盛尿容器要清潔干燥。 使用一次性的容器(如塑料尿杯),避免因用藥并清洗不干凈而造成的污染,影響檢 測結果。尿液標本必須新鮮,留取后,應及時檢測或保存,以免細菌繁殖。因在室溫中久 置后(尤其是夏季)尿中磷酸鹽等可析出結晶而干擾檢測。
● 標本宜在新鮮時檢測。如有細菌污染,菌體中可能含有內源性 HRP,也會產生假陽性反應。 保存過久可發生聚合在 ELISA 中可使本底加深。
● 凍結標本融解后,蛋白質局部濃縮,分布不均,應充分混勻宜輕緩,避免氣泡,可上下顛 倒混和,不要在混勻器上強烈振蕩。
● 混濁或有沉淀的標本應先離心或過濾,澄清后再檢測。
● 反復凍融會使蛋白效價降低,所以待測標本如需保存作多次檢測,宜少量分裝冰存。也可 加入適當防腐劑。
● 如果您在實驗中遇到任何特殊問題,敬請咨詢我公司深圳子科生物技術部。
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