小鼠癌胚抗原(CEA/CD66)ELISA試劑盒子科生物免費代測,2016年年中*!進行詢價,全場可以享受7折優惠!并可享受滿減活動,滿10000減1000,滿5000減200元,滿1000元減50元,期待與您的合作!
[產品名稱]小鼠癌胚抗原(CEA/CD66)ELISA試劑盒
[英文名稱]Mouse carcinoembryonic antign,CEA ELISA Kit
[貨號]ZK-M4906
[試劑盒]保存條件、保質期2-8℃低溫保存下,6個月的保質期。
[運輸方式]當地客戶可以由專門配送人員免費送貨上門貨快遞,外地客戶當天下單當天免費快遞送貨上門。
[技術服務]凡購買子科生物子科生物任何一款ELISA酶聯免疫分析檢測試劑盒,都可以享受免費代測服務,外地客戶可以由技術老師的指導下正確放置好樣本的保存方式后快遞郵寄到我公司技術部,本地客戶可以享受免費上門取樣服務!
[產品價格]詳細價格可以咨詢在線客服2811391951,2016年年中*!或 進行詢價,可以享受7折優惠!
●小鼠癌胚抗原(CEA/CD66)ELISA試劑盒標本的采集及保存:
1.血清:標本請于室溫放置2小時或4℃過夜后于1000 x g離心20分鐘,取上清即可檢測,或將標本放于-20℃保存,但應避免反復凍融。
2.血漿:可用EDTA或肝素作為抗凝劑,標本采集后30分鐘內于2 - 8° C 1000 x g離心15分鐘,或將標本放于-20℃保存,但應避免反復凍融。
●操作步驟
各試劑在使用前平衡至室溫。
1. 加樣:分別設空白孔、標準孔、待測樣品孔。除空白孔外,余孔分別加標準溶液或待測樣品100ul,注意不要有氣泡,輕輕混勻,酶標板加上蓋,37℃反應120分鐘。
2. 棄去液體,甩干,不用洗滌。
3. 每孔加檢測溶液A工作液 100ul,37℃,60分鐘。洗板3次,350ul/每孔,甩干。
4. 每孔加檢測溶液B工作液 100ul,37℃,60分鐘,洗板5次,甩干。
5. 依序每孔加底物溶液90ul,37℃避光顯色30分鐘(此時肉眼可見標準品的前3-4孔有明顯的梯度蘭色,后3-4孔梯度不明顯)。
6. 依序每孔加終止溶液50ul,終止反應(此時蘭色立轉黃色)。用酶聯儀在450nm波長依序測量各孔的光密度(OD值)。
●注:1.每次實驗留一孔作為空白調零孔,該孔不加任何試劑,只是zui后加底物溶液及2NH2SO4。測量時先用此孔調OD值至零。
2.為防止樣品蒸發,試驗時將反應板放于鋪有濕布的密閉盒內。
●洗板方法:
手工洗板方法:吸去(不可觸及板壁)或甩掉酶標板內的液體;在實驗臺上鋪墊幾層吸水紙,酶標板朝下用力拍幾次;將推薦的洗滌緩沖液至少0.4ml注入孔內,浸泡1-2分鐘,根據需要,重復此過程數次。
自動洗板:如果有自動洗板機,應在熟練使用后再用到正式實驗過程中。
其他相關產品如下:Total Hel-N1 Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total FoxC1/2 Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total FoxI1 Cell-Based Fluorometric ELISA Kit Human, Mouse 96 well Immunoway
Total FoxL1 Cell-Based Fluorometric ELISA Kit Human 96 well Immunoway
Total CAF-1 p60 Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total MKP-2 Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total TIEG-1/2 Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total PSMD2 Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total TBX2 Cell-Based Fluorometric ELISA Kit Human, Mouse 96 well Immunoway
Total cGKII Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total SRp40 Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total p39 Cell-Based Fluorometric ELISA Kit Human 96 well Immunoway
Total MTA1 Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total EKLF/CKLF/UKLF Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total JIP-2 Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total Treacle Cell-Based Fluorometric ELISA Kit Human, Mouse 96 well Immunoway
Total N33 Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total FoxE3 Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total PRDM3 Cell-Based Fluorometric ELISA Kit Human 96 well Immunoway
Total Smad4 Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total Srb7 Cell-Based Fluorometric ELISA Kit Human, Mouse 96 well Immunoway
Total TDE1 Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total CaMKIIβ/γ Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total ITPK1 Cell-Based Fluorometric ELISA Kit Human, Mouse 96 well Immunoway
Total DGK-ζ Cell-Based Fluorometric ELISA Kit Human 96 well Immunoway
Total Tra-2α Cell-Based Fluorometric ELISA Kit Human, Mouse 96 well Immunoway
Total Dyrk1A Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total Brk Cell-Based Fluorometric ELISA Kit Human, Mouse 96 well Immunoway
Total TFIIH p44 Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total GIT2 Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total E2F-2 Cell-Based Fluorometric ELISA Kit Human, Mouse 96 well Immunoway
Total EFP Cell-Based Fluorometric ELISA Kit Human 96 well Immunoway
Total GCKR Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Total GK1/3 Cell-Based Fluorometric ELISA Kit Human 96 well Immunoway
Total GK2 Cell-Based Fluorometric ELISA Kit Human 96 well Immunoway
Total Mad 4 Cell-Based Fluorometric ELISA Kit Human, Mouse 96 well Immunoway
Total KIAA0100 Cell-Based Fluorometric ELISA Kit Human 96 well Immunoway
Total MELK Cell-Based Fluorometric ELISA Kit Human 96 well Immunoway
Total Mi2-β Cell-Based Fluorometric ELISA Kit Human, Mouse 96 well Immunoway
Total NP220 Cell-Based Fluorometric ELISA Kit Human 96 well Immunoway
Total MB67 Cell-Based Fluorometric ELISA Kit Human 96 well Immunoway
Total FASTKD3 Cell-Based Fluorometric ELISA Kit Human 96 well Immunoway
Total WTAP Cell-Based Fluorometric ELISA Kit Human, Mouse 96 well Immunoway