小鼠血管內皮生長因子(VEGF)ELISA試劑盒子科生物現貨供應,凡購買子科生物子科生物任何一款ELISA酶聯免疫分析檢測試劑盒,都可以享受免費代測服務,外地客戶可以由技術老師的指導下正確放置好樣本的保存方式后快遞郵寄到我公司技術部,本地客戶可以享受免費上門取樣服務!
?產品名稱:小鼠血管內皮生長因子(VEGF)ELISA試劑盒
?英文名稱:Mouse vascular endothelial growth factor ELISA kit
?產品規格:96T(人份)/48T(人份)(單孔檢測分別可以檢測90份樣本,42分樣本,一般都需要留6個孔做標準曲線)。
?檢測方法:酶聯免疫分析ELISA方法
?有效期: 4 個月(4℃);8 個月(-20℃)。
?保存方法: 2-8℃(頻繁使用時); -20℃(長時間不用時)。
?品牌:子科生物ZIKER,美國R&D,美國immonoway,美國sciencell,德國IBL
?質量保證:我告訴所有產品均需要經過質檢通過后,才允許出庫!
?適用范圍:實驗結果僅供科研參考,不推薦用于臨床診斷結果。
?ELISA常用的方法:雙抗體夾心法、間接法、競爭法以及BAS-ELISA等,子科生物ELISA產品主要用雙抗體夾心法。
小鼠血管內皮生長因子(VEGF)ELISA試劑盒實驗說明:
1.試劑盒保存:-20℃(較長時間不用時);2-8℃(頻繁使用時)。
2.濃洗滌液低溫保存會有鹽析出,稀釋時可在水浴中加溫助溶。
3.中、英文說明書可能會有不*之處,請以英文說明書為準。
4.剛開啟的酶聯板孔中可能會含有少許水樣物質,此為正常現象,不會對實驗結果造成任何影響。
深圳子科生物科技有限公司專業研發生產酶聯免疫ELISA試劑盒,ELISA 檢測的目的是為實驗提供準確可靠的定量分析實驗依據,為了保證實驗數據的可靠性,在實驗過程中必須堅持全面質量控制和全過程質量控制。在收集標本前都必須有一個完整的計劃。
子科生物技術部總結介紹如下:
注意事項:
●每個標本量收集體積= 100ul × 檢測種類,如要做復孔,標本量收集體積= 100ul × 檢測 種類×2 。
● 對于作某一個具體指標來說,先用一系列稀釋度作一批標本測定,得出對實驗有意義 的一個稀釋度(即測定劑量反應曲線),然后用一個zui適稀釋度測定即可。
● 收集標本前必須清楚要檢測的成份是否足夠穩定。對收集后當天進行檢測的標本,儲存在 4 ℃ 備用,如有特殊原因需要周期收集標本,將標本及時分裝后放在 -20 ℃ 或 -70 ℃ 條 件下保存。避免反復凍融。標本 2 -8 ℃ 可保存 48 小時, -20 ℃ 可保存 1 個月。 -70 度 可保存 6 個月。部分激素類標本需添加抑肽酶。
● 血清標本采集時,應注意避免溶血,紅細胞溶解時會釋放出具有過氧化物酶活性的物質, 以 HRP 為標記的 ELISA 測定中,溶血標本可能會增加非特異性顯色。
●為了保證尿液檢測結果的準確性,必須正確收集尿液標本和保存。盛尿容器要清潔干燥。 使用一次性的容器(如塑料尿杯),避免因用藥并清洗不干凈而造成的污染,影響檢 測結果。尿液標本必須新鮮,留取后,應及時檢測或保存,以免細菌繁殖。因在室溫中久 置后(尤其是夏季)尿中磷酸鹽等可析出結晶而干擾檢測。
● 標本宜在新鮮時檢測。如有細菌污染,菌體中可能含有內源性 HRP,也會產生假陽性反應。 保存過久可發生聚合在 ELISA 中可使本底加深。
● 凍結標本融解后,蛋白質局部濃縮,分布不均,應充分混勻宜輕緩,避免氣泡,可上下顛 倒混和,不要在混勻器上強烈振蕩。
● 混濁或有沉淀的標本應先離心或過濾,澄清后再檢測。
● 反復凍融會使蛋白效價降低,所以待測標本如需保存作多次檢測,宜少量分裝冰存。也可 加入適當防腐劑。
● 如果您在實驗中遇到任何特殊問題,敬請咨詢我公司深圳子科生物技術部。
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